摘要
茶碱抑制嗜酸性粒细胞向支气管壁的浸润。目前尚不清楚这是否由环磷酸腺苷(c-AMP)依赖性降低支气管上皮细胞嗜酸性粒细胞趋化活性(ECA)介导。因此,我们用白细胞介素(IL)-1 β和肿瘤坏死因子- α (tnf - α)来评估β -2激动剂procaterol和茶碱对BEC细胞系BEAS-2B ECA释放的影响。用盲孔趋化室评估ECA,用酶联免疫吸附试验和逆转录聚合酶链反应评估细胞因子的释放和基因表达。il -1 β和tnf - α以剂量和时间依赖性的方式刺激ECA从BEAS-2B细胞释放。Procaterol和茶碱直接抑制嗜酸性粒细胞向il -1 -和tnf -条件培养液的迁移。同样浓度的procaterol预处理BEAS-2B细胞后,ECA的释放呈剂量依赖性抑制。抗il -8、抗活化调节、正常t细胞表达和分泌(RANTES)、抗粒细胞-巨噬细胞集落刺激因子(GM-CSF)抑制ECA。Procaterol以剂量依赖的方式抑制RANTES、GM-CSF和IL-8的释放。茶碱的作用较弱。 Procaterol augmented cAMP levels in BEAS-2B cells in a time- and dose-dependent manner. The expression of IL-8, RANTES, and GM-CSF messenger ribonucleic acid was not inhibited by procaterol and theophylline. These data indicate that procaterol and theophylline may directly inhibit eosinophil migration and that procaterol may further inhibit the release of eosinophil chemotactic activity from BEAS-2B cells via a cyclic adenosine monophosphate-dependent mechanism. This warrants further studies on the involvement of bronchial epithelial cells in the anti-inflammatory effects of procaterol and theophylline in patients with asthma.