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9.963 144.113 457.795 Tm (,)Tj /F4 1 Tf 0.5918 0 TD (i.p)Tj /F3 1 Tf 1.0243 0 TD [(;)-344.6(Rho)-7(ne)-343.4(Meri)-9.4(eux)-349.8(Australia)-357.3(Pty)]TJ -10.2316 -1.0528 TD [(Ltd.,)-316.8(Pinkem)-9.7(ba,)-315.3(Aus)-7.4(tralia\).)-324.2(A)-312(0)-6.5(.4-mL)-319.6(blood)-319.2(sample)-319.4(was)]TJ 0 -1.0471 TD [(taken)-371.2(to)-362.4(deter)-8.6(mine)-367.5(the)-369.3(level)-9.4(s)-360.5(o)0(f)-355.9(free)-371.2(CGS)-368.7(26303)-368(in)-368.1(the)]TJ 0 -1.0527 TD [(circu)-8.6(lation.)-382.4(Plasm)-10.2(a)-376.7(samp)-7.8(les)-381.8(wer)-8.2(e)-376.7(centr)-8.6(ifuged)-384.5(in)-379.5(Cen)-7.4(tri-)]TJ 0 -1.0471 TD [(free)-268.8(tubes)-269.6(\(Amic)-9.6(on,)-263.7(Beverly)53.4(,)-262.1(M)0(A)-7.4(,)-262.1(USA\))-267.8(and)-270.2(the)-266.8(concen-)]TJ 0 -1.0527 TD [(trations)-494.4(of)-486.8(CGS)-488.2(26303)-487.5(in)-487.6(the)-483.1(fil)-8.6(trates)-490.9(were)-487.4(meas)-8.2(ured)]TJ 0 -1.0471 TD [(using)-565.1(a)-558.8(neutral)-564.5(endopept)-9.3(idase)-565.9(24.1)31.5(1)-558.4(inhi)-7.8(bition)-563.7(assay)55.6(.)]TJ 0 -1.0528 TD [(Lungs)-305(wer)-8.2(e)-302.8(blotted)-308.8(dry)58.7(,)-296.3(wei)-9(ghed)-305.1(and)-304.3(prepare)-9.7(d)-296.7(for)-307.1(deter-)]TJ 0 -1.0471 TD [(minati)-9.6(on)-416.9(of)-418.5(lung)-420.8(viral)-426(titres)-424.6(and)-418.1(ex)-7.6(traction)-425.4(of)-418.5(ET)69.9(.)-415.8(The)]TJ 0 -1.0528 TD [(trach)-8.6(ea)-264.1(from)-271.2(each)-266(animal)-269.4(was)-269.6(careful)-12.1(ly)-260(cleane)-8.6(d)-268.2(o)0(f)-264.9(adher-)]TJ 0 -1.0471 TD [(ent)-466(fat)-467.6(and)-469.3(connect)-8.9(ive)-466(tissue)-473.1(and)-463.6(prepar)-8.6(ed)-468.6(for)-466.4(autor)-8.2(a-)]TJ 0 -1.0527 TD [(diogr)-7.8(aphy)59.1(.)]TJ /F4 1 Tf 0 -2.5551 TD [(Lung)-338(virus)-339(titr)28.4(es)]TJ /F3 1 Tf 1.0015 -1.6503 TD [(Lu)17.1(ng)-405.6(ti)16.5(ss)18.9(ue)20.8(s)-417.4(w)0(e)16.9(r)0(e)-407.6(h)0(o)21.2(m)0(o)12.4(g)0(e)20.8(n)0(i)18.9(z)0(e)14.7(d)-410.5(in)-407.9(st)17.7(er)19.2(il)16.5(e)-416.6(s)0(a)19.6(l)0(i)16.5(n)0(e)-406(w)0(i)14.9(t)0(h)]TJ -1.0015 -1.0471 TD [(gl)18.9(as)13.9(s/)17.7(gl)18.9(as)19.6(s)-297.9(t)0(i)16.5(s)0(s)18.9(u)0(e)-292.2(h)0(o)21.2(m)0(o)12.4(g)0(e)20.8(n)0(i)18.9(z)0(e)14.7(r)0(s)18.4(,)-296.3(an)15.1(d)-296.7(t)0(h)18.9(e)-297.1(re)13.5(su)20(lt)16.5(in)18.9(g)-302.4(s)0(u)20(s)0(p)20(e)0(n)15.1(-)]TJ 0 -1.0528 TD [(si)17.7(on)-269(wa)16.9(s)-275.1(c)0(l)18.4(a)0(r)13.5(i)0(f)17.3(i)0(e)18.4(d)-279.6(by)-263.3(ce)14.7(nt)18.9(ri)17.3(fu)13.9(ga)20.8(ti)16.5(on)-269(at)-266.1(2,)15.9(00)21.2(0)]TJ /F10 1 Tf 17.3277 0 TD (6)Tj /F4 1 Tf 0.9902 0 TD (g)Tj /F3 1 Tf 0.7739 0 TD [(fo)13.9(r)-275.5(5)-279.6(mi)15.7(n)]TJ -19.0918 -1.0471 TD [(at)-522.2(4)]TJ /F10 1 Tf 1.7242 0 TD (8)Tj /F3 1 Tf 0.3756 0 TD [(C)11.6(.)-529.6(In)13.9(fe)19.2(ct)18.5(io)13.2(us)-520.6(vi)18.9(ru)13.9(s)-531.2(w)0(a)16.9(s)-531.2(as)19.6(sa)13.9(ye)20.8(d)-535.7(b)0(y)-519.4(a)0(l)18.5(l)0(a)18.4(n)0(t)13.2(o)0(s)20(i)0(s)17.7(-)0(o)19.6(n)0(-)]TJ -2.0998 -1.0527 TD [(sh)20(el)12.8(l)-333.2(t)0(i)16.5(t)0(r)17.3(a)0(t)18.4(i)0(o)18.9(n)-336.5(fo)19.6(r)-338.1(i)0(n)18.9(f)0(e)19.2(c)0(t)18.4(i)0(v)13.2(i)0(t)16.5(y)-330.8(as)-327.5(pr)19.6(ev)15.1(io)18.9(us)20(ly)-328.3(de)15.1(sc)19.6(ri)17.3(be)20.8(d)-336.5([)0(1)19.6(0].)]TJ 0 -1.0471 TD [(Briefl)-12(y)61.8(,)-410.1(6)]TJ /F10 1 Tf 3.898 0 TD (6)Tj /F3 1 Tf 1.0015 0 TD [(6)-416.2(m)0(m)-417.5(pieces)-419.1(of)-412.8(allantoi)-10.7(s-on-)-8.2(shell)-414.2(from)-419.1(1)27.7(1)0(-)]TJ -4.8996 -1.0528 TD [(day-ol)-9(d)-518.6(embryonate)-10.3(d)-518.6(chicke)-8.2(n)-518.6(eggs)-522.5(were)-521.5(incuba)-7.8(ted)-522.9(in)]TJ T* [(sterile)-721.7(round)-722.4(bott)-7.8(om)-716(tubes)-719.1(contai)-10.1(ning)-716.8(0.35)-719.7(mL)-720.1(of)]TJ 0 -1.0471 TD [(Standar)-9.3(d)-512.9(Medi)-7.8(um)-511.1(\(SM\))-519.6(and)-514.8(25)]TJ /F9 1 Tf 13.9874 0 TD (m)Tj /F3 1 Tf 0.5747 0 TD [(L)-517(aliquots)-521.3(of)-509.6(seri)-8.6(al)]TJ -14.5621 -1.0527 TD [(10-fol)-10.1(d)-518.6(d)-6.5(ilutions)-529(of)-526.7(virus)-526.8(\(10)]TJ 7.472 0 0 6.641 184.025 211.124 Tm (-2)Tj 9.963 0 0 9.963 195.42 206.646 Tm [(to)-527.4(10)]TJ 7.472 0 0 6.641 218.324 211.124 Tm (-6)Tj 9.963 0 0 9.963 224.447 206.646 Tm [(\))-525.9(i)0(n)-527.4(SM.)-526.6(Five)]TJ -16.679 -1.0471 TD [(replicate)-11.5(s)-326.3(wer)-8.2(e)-325.5(used)-334.7(at)-328.7(each)-328.6(dilu)-7.8(tion.)-332.6(T)29.3(ubes)-329(wer)-8.2(e)-325.5(seale)-8.6(d)]TJ 0 -1.0527 TD [(and)-230.3(placed)-235.8(in)-225.8(a)-228.8(wor)-7.8(king)-227.4(h)-6.5(orizontal)-238.3(shaker)-230.1(in)-231.5(a)-228.8(3)0(5)]TJ /F10 1 Tf 19.4218 0 TD (8)Tj /F3 1 Tf 0.387 0 TD [(C)-227.4(room)]TJ -19.8088 -1.0471 TD [(for)-369.7(48)-365.7(h.)-365.3(The)-371(fluid)-368.7(from)-373.6(each)-368.5(tube)-370(was)-372(transferr)-11.9(ed)-366.1(to)-368.1(a)]TJ 0 -1.0528 TD [(haema)-8.2(gglutinat)-11.1(ion)-505.4(tray)-513.9(and)-503.5(one)-509.2(drop)-511.1(of)-503.9(10)-7.2(%)-503.9(was)-7.8(hed)]TJ 0 -1.0471 TD [(goose)-324.1(red)-323(blood)-324.9(ce)-8(lls)-321.2(was)-326.5(added)-323.3(to)-322.6(each)-322.9(wel)-9(l.)-322.2(The)-319.8(trays)]TJ 0 -1.0528 TD [(were)-373.6(shaken)-370.8(and)-366.9(left)-374(to)-368.1(stand)-372(for)-369.7(40)-365.7(min)-7.8(.)-364.6(Posit)-9(ive)-369.3(hae-)]TJ 0 -1.0471 TD [(maggl)-9.7(utination)-517.6(indicated)-516(infec)-8.6(tion)-508.6(and)-509.2(the)-511.5(EI)-7.2(D)]TJ 7.472 0 0 6.641 257.499 131.811 Tm (50)Tj 9.963 0 0 9.963 270.028 133.455 Tm (was)Tj -21.2542 -1.0528 TD [(calcul)-10.6(ated)-336.3(by)-343(the)-335.1(met)-8.2(hod)-338(of)-338.9(T)]TJ 6.974 0 0 6.974 181.02 122.967 Tm 0.0001 Tc (HOMSON)Tj 9.963 0 0 9.963 214.469 122.967 Tm 0 Tc [([1)31(1].)-339.2(The)-342.6(compo)-7.4(-)]TJ -15.6775 -1.0471 TD [(sition)-246.2(of)-236.4(the)-244.1(SM)-236(\(pH)-241.1(7)-6.5(.0\))-236.8(was)-241.1(\(in)-239.6(mM\))-9(:)-236.5(NaCl)-247.8(137,)-236(KCl)-246.6(8,)]TJ 25.1977 66.1583 TD (CaCl)Tj 7.472 0 0 6.641 329.726 770.003 Tm (2)Tj 9.963 0 0 9.963 336.189 771.647 Tm [(7.2,)-275.4(MgCl)]TJ 7.472 0 0 6.641 377.064 770.003 Tm (2)Tj 9.963 0 0 9.963 383.527 771.647 Tm [(0.52,)-276.2(glucos)-8.6(e)-274.3(1.7,)-275.4(acid-)-8.6(free)-280.2(gelatin)-280.8(2.0)]TJ -7.4489 -1.0471 TD (g)Tj /F8 1 Tf 0.5008 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (L)Tj 7.472 0 0 6.641 323.093 765.638 Tm (-1)Tj 9.963 0 0 9.963 329.216 761.216 Tm [(,)-262.1(phenol)-269.1(red)-260.4(2.5)-263.7(mg)]TJ /F8 1 Tf 7.7961 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (L)Tj 7.472 0 0 6.641 415.616 765.638 Tm (-1)Tj 9.963 0 0 9.963 421.795 761.216 Tm [(,)-262.1(penicillin)-270.7(5)-262.5(UmL)]TJ 7.472 0 0 6.641 495.212 765.638 Tm (-1)Tj 9.963 0 0 9.963 501.335 761.216 Tm [(,)-262.2(strepto-)]TJ -19.2739 -1.0528 TD [(myci)-9(n)-330.8(5)]TJ /F9 1 Tf 3.6533 0 TD (m)Tj /F3 1 Tf 0.5747 0 TD (g)Tj /F8 1 Tf 0.5008 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (mL)Tj 7.472 0 0 6.641 372.869 755.149 Tm (-1)Tj 9.963 0 0 9.963 382.337 750.727 Tm [(and)-332.7(ampho)-7.4(teric)-11(in)-334(B)-335.5(12.5)-332.7(ng)]TJ /F8 1 Tf 11.4209 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (mL)Tj 7.472 0 0 6.641 512.56 755.149 Tm (-1)Tj 9.963 0 0 9.963 518.74 750.727 Tm (.)Tj /F4 1 Tf -21.0209 -2.555 TD [(Aut)-8.8(oradiograph)-7.5(ic)-334.4(studi)-9.8(es)]TJ /F3 1 Tf 1.0015 -1.6503 TD [(T)34.9(r)0(a)19.2(c)0(h)15.1(e)0(a)20.4(l)-304.7(tu)13.2(be)20.8(s)-309.2(w)0(e)16.9(r)0(e)-299.4(s)0(u)20(b)0(m)18.1(e)0(r)24.9(g)0(e)15.1(d)-302.4(in)-299.8(Ma)18.9(cr)13.5(od)21.2(ex)-297.8(\(6)13.9(%)-304.7(d)0(e)15.1(x)0(-)]TJ -1.0015 -1.0471 TD [(tr)17.3(an)-223.9(70)-217.8(in)-214.5(5%)-220.1(gl)13.2(uc)20.8(os)14.4(e\))-219.8(an)20.8(d)-228.4(f)0(r)18(o)0(z)15.1(e)0(n)-218.2(b)0(y)-217.8(i)0(m)15.7(m)0(e)17.7(r)0(s)18.4(i)0(o)18.9(n)-228.4(in)-220.2(is)17.7(op)15.5(en)20.8(-)]TJ 0 -1.0527 TD -0.0158 Tc [(t)-15.8(a)-3(n)-15.8(e)5(,)-608(q)-15.8(ue)-15.8(n)5(c)-15.8(he)-15.8(d)-598.2(w)-15.8(it)-15.8(h)-594.5(l)-15.8(iq)-15.8(ui)-15.8(d)-600.1(n)-15.8(it)-15.8(ro)-15.8(ge)-15.8(n)5(.)-608(Se)-15.8(ri)-15.8(al)-610.8(tr)-15.8(an)-15.8(sv)-15.8(er)-15.8(se)]TJ 0 -1.0471 TD 0 Tc [(se)13.9(ct)18.5(io)18.8(ns)-196.2(\(1)19.6(0)]TJ /F9 1 Tf 4.8313 0 TD (m)Tj /F3 1 Tf 0.5634 0 TD [(m\))-194.1(we)11.2(re)-191.3(cu)20.8(t)-202.3(a)0(t)-197.8(-)0(2)19.6(0)]TJ /F10 1 Tf 6.9652 0 TD (8)Tj /F3 1 Tf 0.3756 0 TD [(C)-204.7(a)0(n)15.1(d)-199.9(th)18.9(aw)16.9(-m)16.5(ou)15.5(nt)18.9(ed)-195.4(on)21.2(to)]TJ -12.7354 -1.0527 TD [(ge)15.1(la)18.4(ti)16.5(n/)18.9(ch)15.1(ro)19.6(me)17.7(al)12.8(um)-255.1(co)20.8(at)18.5(ed)-258(gl)18.9(as)19.6(s)-263.7(m)0(i)15.7(c)0(r)13.5(o)0(s)20(c)0(o)15.1(p)0(e)-252.3(s)0(l)17.7(i)0(d)18.9(e)0(s)13.9(.)-262.1(T)12.2(h)0(e)15.1(s)0(e)]TJ 0 -1.0471 TD [(se)13.9(ct)18.5(io)18.8(ns)-480.7(we)16.9(re)-481.5(pr)19.6(e-)13.5(in)18.9(cu)15.1(ba)20.8(te)18.4(d)-490.2(\()0(2)]TJ /F10 1 Tf 12.6159 0 TD (6)Tj /F3 1 Tf 0.9902 0 TD [(10)-479.5(mi)15.7(n\))-481.1(at)-476.6(22)]TJ /F10 1 Tf 5.9751 0 TD (8)Tj /F3 1 Tf 0.3756 0 TD 0.4892 Tc [(Ci)489.2(n)7.3(a)]TJ -19.9568 -1.0527 TD 0 Tc [(bu)15.5(f)20.4(f)0(e)13.5(r)-395(\(5)19.6(0)-393.4(m)0(M)-387.9(T)40.6(ri)17.3(s-)18.4(HC)12.6(l,)-384.8(10)21.2(0)-393.4(m)0(M)-387.9(N)0(a)16.9(C)0(l)14.1(,)-393(0.)21.6(25)15.5(%)-395.8(b)0(o)15.5(v)0(i)18.9(n)0(e)]TJ T* [(se)13.9(ru)19.6(m)-339.7(a)0(l)12.8(b)0(u)21.2(m)0(i)15.7(n)0(,)-331.2(p)0(H)-329.8(7)0(.)21.6(4)0(\))-333.2(c)0(o)15.1(n)0(t)18.9(a)0(i)18.4(n)0(i)18.8(n)0(g)-331.6(t)0(h)18.8(e)-342.6(pr)19.6(ot)18.9(ea)14.7(se)-327.5(in)13.2(hi)18.9(bi)18.9(to)18.8(r)]TJ 0 -1.0471 TD [(ph)15.5(en)15.1(yl)18.9(me)17.7(th)18.9(ys)14.4(ul)18.9(ph)21.2(on)15.5(yl)18.9(fl)17.3(uo)15.5(ri)17.3(de)-388.9(\(1)19.6(0)]TJ /F9 1 Tf 14.0955 0 TD (m)Tj /F3 1 Tf 0.5634 0 TD [(M\))12(,)-398.7(a)0(n)20.8(d)-399.1(th)18.8(en)-394.6(in)-390.9(an)20.8(-)]TJ -14.6589 -1.0528 TD [(ot)13.2(he)20.8(r)-349.5(b)0(u)15.5(f)20.4(fe)19.2(r)-349.5(c)0(o)15.1(n)0(t)18.9(a)0(i)18.5(n)0(i)18.9(n)0(g)-337.3(0)0(.)15.9(2)-347.9(nM)]TJ 7.472 0 0 6.641 436.819 619.143 Tm [(12)28.8(5)]TJ 9.963 0 0 9.963 447.76 614.664 Tm [(I-)18(ET)104(-1)-344.6(al)18.5(on)21.2(e)-348.3(f)0(o)13.9(r)-343.8(2.)15.9(5)-347.9(h)]TJ -13.8963 -1.0471 TD [(\(t)17.3(ot)13.2(al)-209.2(bi)18.9(nd)15.5(in)18.9(g\))-213.7(or)-208(in)-208.8(th)13.2(e)-217.4(p)0(r)19.6(e)0(s)13.9(e)0(n)20.8(c)0(e)-212.9(o)0(f)-213.7(B)11.6(Q-)15.7(12)15.5(3)-217(\()0(s)18.4(e)0(l)12.8(e)0(c)20.4(t)0(i)16.5(v)0(e)-212.5(E)12.2(T)]TJ 7.472 0 0 6.641 531.099 602.589 Tm (A)Tj 9.963 0 0 9.963 309.316 593.745 Tm [(re)13.5(ce)20.4(pt)13.2(or)-321.8(li)16.5(ga)20.8(nd)15.5(;)-333.2(1)]TJ /F9 1 Tf 7.3579 0 TD (m)Tj /F3 1 Tf 0.5634 0 TD [(M\))-323.7(or)-327.5(sa)19.6(ra)19.2(fo)13.9(to)18.9(xi)18.9(n)-336.5(S)11.4(6c)-320.6(\(s)12.8(el)18.5(ec)20.4(ti)16.5(ve)-326.3(E)12.2(T)]TJ 7.472 0 0 6.641 531.496 592.101 Tm (B)Tj 9.963 0 0 9.963 309.316 583.313 Tm [(re)13.5(ce)20.4(pt)13.2(or)-287.7(li)16.5(ga)20.8(nd)15.5(;)-299.1(1)0(0)15.5(0)-296.7(nM)19.3(\).)-293(No)17.3(ns)20(pe)15.1(ci)18.4(fi)17.3(c)-302.8(b)0(i)18.9(n)0(d)21.2(i)0(n)13.2(g)-296.7(wa)16.9(s)-297.9(d)0(e)15.1(t)0(e)18.5(r)20.4(-)]TJ 0 -1.0528 TD [(mi)15.7(ne)15.1(d)-353.6(i)0(n)-345.3(t)0(h)18.8(e)-354(co)15.1(mb)18.1(in)18.8(ed)-343.4(pr)13.9(es)19.6(en)15.1(ce)-343.8(of)-344.6(BQ)12.6(-1)19.6(23)-343(\(1)]TJ /F9 1 Tf 19.2625 0 TD (m)Tj /F3 1 Tf 0.5634 0 TD [(M\))-346.5(an)15.1(d)]TJ -19.8259 -1.0471 TD [(sa)13.9(ra)19.2(fo)13.9(t)13.9(o)0(x)15.5(i)0(n)-214.5(S)0(6)16.3(c)-223.1(\(1)13.9(00)-212.1(nM)13.6(\).)-213.3(Af)15.7(te)18.4(r)-224.3(2)0(.)15.9(5)-222.7(h,)-217.4(ti)16.5(ss)18.8(ue)-218.2(se)19.6(ct)18.4(io)18.8(ns)-219(we)16.9(re)]TJ 0 -1.0528 TD [(wa)16.9(sh)14.4(ed)-235.2(t)13.9(w)0(i)14.9(c)0(e)-235.7(f)0(o)19.6(r)-241.4(10)-234.8(mi)15.7(n)-239.8(i)0(n)-237.2(b)0(u)21.2(f)14.7(fe)19.2(r)43.2(,)-245.1(ri)17.3(ns)20(ed)-235.2(in)-231.5(di)18.9(st)17.7(il)16.5(le)12.8(d)-239.8(w)0(a)16.9(t)0(e)18.4(r)]TJ 0 -1.0471 TD [(an)15.1(d)-285.3(d)0(r)19.6(i)0(e)12.8(d)-279.6(un)15.5(de)15.1(r)-281.2(a)-285.7(st)17.7(re)13.5(am)-278.2(of)-276.3(co)20.8(ld)-277.1(dr)19.6(y)-285.3(a)0(i)12.8(r)60.2(.)-284.9(E)12.2(mu)18.1(ls)17.7(io)13.2(n-)19.6(co)15.1(at)18.4(ed)]TJ 0 -1.0528 TD [(co)15.1(ve)15.1(r)-486.1(s)0(l)17.7(i)0(p)18.8(s)-485.7(\(K)15.7(od)15.5(ak)-474.3(NT)13.2(B)11.6(-)0(2)13.9(;)-486.8(E)12.2(a)0(s)13.9(t)13.9(m)0(a)12(n)-484.5(Ko)17.3(da)15.1(k)-484.5(C)0(o)16.5(m)0(p)18.1(a)0(n)15.1(y)73.2(,)]TJ 0 -1.0471 TD [(Ro)16.5(ch)15.1(es)19.6(te)18.4(r)37.5(,)-222.3(NY)144.3(,)-222.3(U)0(S)18.1(A)0(\))-217.6(w)12.4(er)13.5(e)-223.1(a)0(t)18.4(t)0(a)18.4(c)0(h)15.1(e)0(d)-212.5(t)13.9(o)-222.7(o)0(n)15.5(e)-223.1(en)20.8(d)-222.7(o)0(f)-213.7(t)0(h)18.9(e)-223.1(gl)18.8(as)19.6(s)]TJ 0 -1.0528 TD [(sl)17.7(id)13.2(es)-452.7(wi)14.9(th)-453.5(cy)15.1(an)20.8(oa)15.1(cr)19.2(yl)13.2(at)18.5(e)-462.1(a)0(d)15.1(h)0(e)20.8(s)0(i)17.7(v)0(e)-457.2(a)0(n)15.1(d)-461.7(in)18.9(cu)15.1(ba)15.1(te)18.4(d)-461.7(f)0(o)14(r)-463.3(3)]TJ 0 -1.0471 TD [(da)15.1(ys)-327.1(at)-328.7(4)]TJ /F10 1 Tf 3.659 0 TD (8)Tj /F3 1 Tf 0.3756 0 TD [(C.)-330.2(T)12.2(h)0(e)-332(e)0(m)17.7(u)0(l)18.9(s)0(i)17.7(o)0(n)15.5(-)0(c)19.2(o)0(a)15.1(t)0(e)18.4(d)-336.5(co)20.8(ve)15.1(rs)18.4(li)16.5(ps)-327.1(we)16.9(re)-327.9(de)15.1(ve)20.8(l-)]TJ -4.0346 -1.0527 TD [(op)15.5(ed)-479.9(\(K)15.7(od)15.5(ak)-479.9(De)11.2(kt)18.9(ol)18.9(,)-489.8(1)0(:)18.9(1)0(;)-487.6(K)12.4(od)15.5(ak)-479.9(Au)17.3(st)17.7(ra)13.5(la)18.4(si)17.7(a)-490.6(P)0(t)14(y)-490.2(Lt)20.4(d.)15.9(,)]TJ 0 -1.0471 TD [(Me)13.2(lb)18.8(ou)15.5(rn)19.6(e,)-308.8(Au)17.3(st)17.7(ra)13.5(li)16.5(a\))-310.8(fo)19.6(r)-321(3)-319.4(mi)15.7(n,)-308.4(ri)17.3(ns)20(ed)-314.9(fo)19.6(r)-321(1)0(5)-308.8(s)-320.6(in)-311.2(di)18.8(lu)18.9(te)]TJ 0 -1.0528 TD [(ac)14.7(et)18.5(ic)-226.2(ac)14.7(id)-225.8(\(2)19.6(%\))-227.4(co)15.1(nt)18.9(ai)18.4(ni)18.9(ng)-229.2(ha)20.8(rd)13.9(en)20.8(er)-225.5(\(I)12.4(lf)17.3(or)19.6(d)-234.1(H)0(y)17.3(p)0(a)15.1(m)0(;)-229(I)0(l)17.3(f)0(o)19.6(r)0(d)]TJ 0 -1.0471 TD [(Im)16.5(ag)15.1(in)18.8(g)-273.9(A)0(u)11.6(s)0(t)17.7(r)0(a)19.2(l)0(i)16.5(a)0(,)-263.3(M)0(e)13.2(l)0(b)18.8(o)0(u)21.2(r)0(n)14(e)0(,)-263.3(A)0(u)17.3(s)0(t)17.7(r)0(a)13.5(l)0(i)16.5(a)0(\))-265.3(a)0(n)15.1(d)-273.9(fi)17.3(xe)20.8(r)-275.5(\()0(I)12.3(l)0(f)17.3(o)0(r)19.6(d)]TJ 0 -1.0528 TD [(Hy)17.3(pa)15.1(m,)-209.2(1:)18.9(4;)-208.8(Il)17.3(fo)13.9(rd)-208(Im)16.5(ag)20.8(in)13.2(g)-217(A)12.4(us)14.4(tr)17.3(al)18.4(ia)18.4(\))-218.6(f)0(o)13.9(r)-212.9(2.)15.9(75)-206.4(mi)15.7(n.)-206(T)34.9(i)0(s)17.7(s)0(u)20(e)]TJ 0 -1.0471 TD [(se)13.9(ct)18.5(io)18.8(ns)-480.7(w)12.4(e)0(r)13.5(e)-484.9(th)18.8(en)-479.9(st)17.7(ai)18.4(ne)20.8(d)-490.2(f)0(o)19.6(r)-486.1(30)-479.5(s)-485.7(w)12.4(it)16.5(h)-490.2(G)12.4(il)16.5(l)]TJ /F10 1 Tf 19.0577 0 TD (')Tj /F3 1 Tf 0.2106 0 TD 0.4856 Tc [(sd)485.6(o)501.1(u)485.6(b)506.8(l)485.6(e)]TJ -19.2682 -1.0527 TD 0 Tc [(st)17.7(re)13.5(ng)21.2(th)-396.6(ha)20.8(em)17.7(at)12.8(ox)21.2(yl)18.9(in)13.2(,)-398.7(d)0(e)20.8(h)0(y)15.5(d)0(r)19.6(a)0(t)18.5(e)0(d)-394.6(i)0(n)-390.9(e)0(t)18.4(h)0(a)15.1(n)0(o)15.5(l)13.9(,)-404.4(c)0(l)18.5(e)0(a)20.4(r)0(e)13.5(d)-399.1(in)]TJ 0 -1.0471 TD [(xy)15.5(le)18.4(ne)-320.6(an)20.8(d)-325.1(m)0(o)12.4(u)0(n)21.2(t)0(e)18.4(d)-330.8(\(D)21.4(eP)15.9(eX)16.9(;)-327.5(B)0(D)18.3(H)-329.1(La)16.7(bo)15.5(ra)19.2(to)18.9(ry)-316.1(Su)16.3(pp)15.5(li)16.5(es)19.6(,)]TJ 0 -1.0527 TD [(Ki)14.9(ls)17.7(yt)18.9(h,)-314.1(Au)11.6(st)17.7(ra)19.2(li)16.5(a\))-316.5(fo)19.6(r)-326.7(l)0(i)16.5(g)0(h)21.2(t)-327.5(m)13.2(i)0(c)12.8(r)0(o)19.6(s)0(c)19.6(o)0(p)15.5(y)73.2(.)]TJ 1.0015 -1.0471 TD [(Au)11.6(t)13.9(o)0(r)14(a)0(d)20.8(i)0(o)13.2(g)0(r)19.6(a)0(p)15.1(h)0(i)18.9(c)-240.2(gr)19.6(ai)18.5(n)-239.8(d)0(e)20.8(n)0(s)14.4(i)0(t)16.5(i)0(e)18.4(s)-235.3(we)11.2(re)-225.5(de)15.1(te)18.4(rm)16.5(in)18.9(ed)-229.6(us)14.4(in)18.8(g)]TJ -1.0015 -1.0528 TD [(a)-388.1(c)0(o)20.8(m)0(p)18.1(u)0(t)13.2(e)0(r)30.6(-)0(a)13.5(s)0(s)18.8(i)0(s)17.7(t)0(e)18.4(d)-382(gr)13.9(ai)18.4(n)-382(d)0(e)15.1(t)13.9(ec)14.7(ti)16.5(on)-371.4(an)15.1(d)-382(c)0(o)15.1(u)0(n)21.2(t)0(i)16.5(n)0(g)-377.1(s)0(y)20(s)0(t)17.7(e)0(m)]TJ 0 -1.0471 TD [([1)13.9(2].)-475.8(A)-488.4(total)-489.4(of)-486.8(twelve)-492.1(sli)-8.2(des)-487.6(were)-487.4(asses)-8.2(sed)-487.6(\(4)]TJ /F10 1 Tf 20.0478 0 TD (6)Tj /F3 1 Tf 1.0015 0 TD (total)Tj -21.0493 -1.0528 TD [(bindi)-8.6(ng,)-189.7(3)]TJ /F10 1 Tf 3.9891 0 TD (6)Tj /F3 1 Tf 1.0015 0 TD [(BQ-123)-9.1(,)-182.5(3)]TJ /F10 1 Tf 4.1484 0 TD (6)Tj /F3 1 Tf 1.0015 0 TD [(saraf)-9(otoxin)-191.4(S6c)-189.7(and)-184.8(2)]TJ /F10 1 Tf 8.4448 0 TD (6)Tj /F3 1 Tf 1.0015 0 TD [(BQ-12)-8.4(3)]TJ -19.5869 -1.0471 TD [(and)-418.1(saraf)-9(otoxin)-419.1(S6c\))-414(a)-6.9(n)0(d)-411.3(each)-419.7(slide)-419.9(contained)-422.6(one)-418.1(tra-)]TJ 0 -1.0527 TD [(che)-8.8(al)-231.9(ring)-234.7(from)-242.7(each)-237.6(of)-230.7(32)-234.8(mice)-237(studi)-9.8(ed)-229.6(\()]TJ /F4 1 Tf 16.2181 0 TD (i.e.)Tj /F3 1 Tf 1.4454 0 TD [(eight)-236.6(mic)-8.2(e)-228.8(i)0(n)]TJ -17.6635 -1.0471 TD [(each)-311.6(of)-304.7(the)-312.4(four)-296.5(groups)-7.4(\).)-304.3(Fo)-6.4(ur)-304.7(estimat)-11.9(es)-304.7(of)-310.4(grain)-309.8(density)]TJ 0 -1.0527 TD [(wer)-8.2(e)-553.2(made)-553.3(pe)-7.6(r)-548.7(trach)-8.6(eal)-557.5(ring;)-556.5(three)-554.9(over)-557(the)-557.1(tracheal)]TJ 0 -1.0471 TD [(smoo)-7.4(th)-265.7(musc)-7.8(le)-271.8(band)-265.2(and)-270.2(one)-270.1(over)-272.5(a)-262.9(nonti)-8.6(ssue)-267.6(area)-273.3(in)-265.7(the)]TJ 0 -1.0527 TD [(airw)-11.4(ay)-320.6(lumen.)-328.8(Thus,)-327.4(a)-319.8(total)-324.3(of)-327.5(1,536)-322.1(fields)-331.2(were)-328.1(analys-)]TJ T* [(ed)-366.1(\(12)-368.1(slides)]TJ /F10 1 Tf 5.2467 0 TD (6)Tj /F3 1 Tf 1.0015 0 TD [(32)-365.7(sections)]TJ /F10 1 Tf 4.5581 0 TD (6)Tj /F3 1 Tf 1.0015 0 TD [(4)-365(fields\).)-368.1(Aut)-8.6(oradiographi)-12.2(c)]TJ -11.8079 -1.0471 TD [(grain)-474.8(densi)-7.8(ties)-470.3(are)-465.6(exp)-8.4(ressed)-470.3(as)-469.8(grains)]TJ /F8 1 Tf 16.1896 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (1000)Tj /F9 1 Tf 2.4697 0 TD (m)Tj /F3 1 Tf 0.5747 0 TD (m)Tj 7.472 0 0 6.641 511.37 284.372 Tm (-2)Tj 9.963 0 0 9.963 522.142 279.95 Tm (and)Tj -21.3623 -1.0528 TD [(presen)-8.2(ted)-340.8(as)-333.2(the)-335.1(mean)-342.8(grain)-338.2(density\213)]TJ 6.974 0 0 6.974 459.723 269.461 Tm 0.0001 Tc (SEM)Tj 9.963 0 0 9.963 474.009 269.461 Tm 0 Tc (.)Tj /F4 1 Tf -16.531 -2.5551 TD [(Extract)-10.5(ion)-334.7(of)-339.6(endothelin)-339.9(fr)29(om)-341.2(lung)-335.5(tissue)]TJ /F3 1 Tf 1.0015 -1.6503 TD [(Lu)17.1(ng)15.5(s)-343.4(w)12.4(er)13.5(e)-342.6(h)0(o)21.2(m)0(o)12.4(g)0(e)20.8(n)0(i)18.9(z)0(e)14.7(d)-342.2(wi)14.9(t)13.9(h)-342.2(gl)13.2(as)19.6(s/)17.7(gl)18.9(as)13.9(s)-337.7(t)0(i)16.5(s)0(s)18.8(u)0(e)-337.7(h)0(o)21.2(m)0(-)]TJ -1.0015 -1.0471 TD [(og)15.5(en)15.1(i)13.9(z)0(e)14.7(r)0(s)-465.3(i)0(n)-464.8(a)-473.5(bu)15.5(f)20.4(f)0(e)13.5(r)-474.7(co)20.8(nt)18.9(ai)12.8(ni)18.9(ng)-462.5(1M)-470.1(ac)20.4(et)18.4(ic)-470.9(ac)20.4(id)-464.8(an)15.1(d)-473.1(1)]TJ /F9 1 Tf 0 -1.0527 TD (m)Tj /F3 1 Tf 0.5691 0 TD (g)Tj /F8 1 Tf 0.4894 0.2049 TD (.)Tj /F3 1 Tf 0.2675 -0.2049 TD (mL)Tj 7.472 0 0 6.641 336.076 211.124 Tm (-1)Tj 9.963 0 0 9.963 346.79 206.646 Tm [(pe)15.1(ps)20(ta)12.8(ti)22.2(n)-473.1(A)-471.3(at)-465.2(a)-467.8(r)0(a)13.5(t)0(i)22.2(o)-473.1(of)-464.1(10)-462.5(mL)-464(bu)21.2(f)20.4(f)0(e)13.5(r)]TJ /F8 1 Tf 16.4002 0.2049 TD (.)Tj /F3 1 Tf 0.2618 -0.2049 TD 0.4674 Tc [(gw)467.4(e)484.3(t)]TJ -20.4234 -1.0471 TD 0 Tc [(we)16.9(ig)13.2(ht)]TJ 7.472 0 0 6.641 335.679 200.636 Tm (-1)Tj 9.963 0 0 9.963 344.183 196.214 Tm [(of)-236.4(ti)22.2(ss)13.2(ue)20.8(.)-245.1(L)0(u)17.1(n)0(g)-234.8(h)0(o)15.5(m)0(o)18.1(g)0(e)15.1(n)0(a)20.8(t)0(e)18.4(s)-246.7(we)16.9(re)-236.8(th)18.9(en)-235.2(in)13.2(cu)20.8(ba)15.1(te)18.4(d)]TJ -3.4997 -1.0527 TD [(in)-260(a)-268.6(b)0(o)21.2(i)0(l)16.5(i)0(n)13.2(g)-262.5(wa)16.9(te)12.8(r)-264.1(b)0(a)15.1(t)0(h)-254.3(f)0(o)13.9(r)-264.1(10)-263.3(mi)21.4(n)-268.2(t)0(o)-260(i)0(n)18.9(a)0(c)14.7(t)0(i)16.5(v)0(a)20.8(t)0(e)-260.4(p)0(r)19.6(o)0(t)18.8(e)0(o)15.1(l)0(y)18.9(t)0(i)16.5(c)]TJ 0 -1.0471 TD [(en)15.1(zy)15.1(m)13.2(e)0(s)13.9(,)-239.4(co)20.8(ol)13.2(ed)-229.6(to)-231.5(4)]TJ /F10 1 Tf 8.2285 0 TD (8)Tj /F3 1 Tf 0.3756 0 TD [(C)-238.8(a)0(n)15.1(d)-239.8(ce)14.7(nt)18.9(ri)17.3(fu)19.6(ge)15.1(d)-239.8(a)0(t)-231.9(1)0(0)21.2(0)0(,)15.9(0)0(0)21.2(0)]TJ /F10 1 Tf 11.3413 0 TD (6)Tj /F3 1 Tf 0.9902 0 TD 0.2398 Tc [(gf)239.8(o)259.4(r)]TJ -20.9355 -1.0528 TD 0 Tc [(20)-257.6(mi)15.7(n.)-257.2(Th)17.1(e)-268.6(r)0(e)19.2(s)0(u)14.4(l)0(t)16.5(i)0(n)18.8(g)-268.2(su)20(pe)15.1(rn)19.6(at)18.4(an)15.1(ts)-255.5(we)16.9(re)-259.6(fr)18(oz)15.1(en)-258(an)15.1(d)-262.5(s)0(t)17.7(o)0(r)13.9(e)0(d)]TJ 0 -1.0471 TD [(at)-442.5(-8)19.6(5)]TJ /F10 1 Tf 2.4412 0 TD (8)Tj /F3 1 Tf 0.3756 0 TD [(C.)-438.3(ET)-374(wa)16.9(s)-445.8(e)0(x)15.1(t)0(r)17.3(a)0(c)14.7(t)13.9(ed)-440.1(fr)18(om)-437.2(ti)16.5(ss)18.8(ue)-440.1(su)20(pe)15.1(rn)19.6(at)18.4(an)15.1(ts)-437.6(as)]TJ -2.8168 -1.0528 TD [(de)15.1(sc)19.6(ri)17.3(be)15.1(d)-199.9(p)0(r)13.9(e)0(v)20.8(i)0(o)13.2(u)0(s)20(l)0(y)-191.7([)0(1)13.9(3].)-202.7(Briefl)-12(y)61.8(,)-210.9(C18)-211.9(Se)-6.8(p-Pak)-215.6(cartridges)]TJ 0 -1.0471 TD [(\(W)69.7(a)-6.9(ters,)-316.3(Milford,)-317.2(MA)-7.4(,)-313.4(USA\))-313.3(wer)-8.2(e)-314.2(pretreated)-324.5(with)-313.3(5)-313.7(m)0(L)]TJ 0 -1.0528 TD [(90%)-294.9(acetonitr)-11.1(ile)-286.3(\(ACN)-11.5(\))-286.9(i)0(n)-288.4(1)0(%)-288.4(trifluoroacet)-14(ic)-288.8(acid)-290.8(\(TF)66.8(A\))]TJ 0 -1.0471 TD [(foll)-9.4(owed)-314.7(by)-314.5(5)-313.8(m)0(L)-316.1(25%)-311.9(ACN)-322.2(in)-311.2(1%)-316.9(TF)74.8(A.)-318.1(Sample)-10.1(s)-309.2(\(100)]TJ 8.966 0 0 8.966 527.527 797.499 Tm (93)Tj 7.472 0 0 7.472 206.305 797.499 Tm 0.0001 Tc 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TD (mg)Tj /F8 1 Tf 1.2519 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (kg)Tj 7.472 0 0 6.641 83.282 336.019 Tm -0.0185 Tc (-1)Tj /F8 1 Tf 9.963 0 0 9.963 89.235 333.581 Tm 0 Tc (.)Tj /F3 1 Tf 0.2675 -0.1992 TD [(da)15.1(y)]TJ 7.472 0 0 6.641 105.959 336.019 Tm (-1)Tj 9.963 0 0 9.963 117.581 331.597 Tm [(fo)13.9(r)-560(6)-564.1(da)20.8(ys)14.4(\),)-549(th)13.2(e)-558.8(p)0(l)18.9(a)0(s)13.9(m)0(a)-551.4(l)0(e)18.4(v)0(e)15.1(l)0(s)-551.4(o)0(f)-555.1(C)0(G)18.3(S)]TJ -5.9523 -1.0471 TD [(26)15.5(30)21.2(3)-353.6(i)0(n)-345.3(s)0(h)14.4(a)0(m)17.7(-)0(i)17.3(n)0(o)15.5(c)0(u)20.8(l)0(a)18.4(t)0(e)12.8(d)-353.6(mi)15.7(ce)-343.8(\(2)13.9(57)-343(nM)19.3(;)-356(9)0(5)15.5(%)-355.9(C)11.6(I)0(,)-349.9(1)0(9)21.2(0)0(\261)]TJ 0 -1.0528 TD [(35)15.5(7)-234.1(n)0(M)19.3(\))-241.4(we)16.9(re)-225.5(no)15.5(t)-236.5(s)0(i)17.7(g)0(n)15.5(i)0(f)17.3(i)0(c)18.4(a)0(n)15.1(t)13.9(ly)-231.5(di)18.9(f)20.4(f)0(e)13.5(r)0(e)19.2(n)0(t)-231.5(f)0(r)18(o)0(m)-226.6(l)0(e)18.4(v)0(e)15.1(l)0(s)-227(m)0(e)17.7(a)0(s)13.9(-)]TJ 0 -1.0471 TD [(ur)19.6(ed)-269.4(in)-271.4(vi)18.9(ru)19.6(s-)12.8(in)18.9(oc)20.8(ul)13.2(at)18.4(ed)-269.4(mi)15.7(ce)-269.8(\(1)19.6(62)-269(nM)19.3(;)-282(9)0(5)21.2(%)-282(C)11.6(I)0(,)-270.2(1)0(0)15.5(5)0(\261)21.2(2)0(5)15.5(1)]TJ 0 -1.0527 TD [(nM)19.3(;)]TJ 6.974 0 0 6.974 77.556 289.757 Tm 0.0001 Tc (NS)Tj 9.963 0 0 9.963 86.287 289.757 Tm 0 Tc [(\).)-287.3(Th)17.1(us)20(,)-296.3(t)0(h)18.9(e)-297.1(pl)18.9(as)13.9(ma)-283.9(le)12.8(ve)20.8(ls)-289.6(of)-287.7(C)11.6(G)0(S)-289.2(2)0(6)15.5(3)0(0)21.2(3)-296.7(ac)14.7(hi)18.9(ev)20.8(ed)]TJ -2.8111 -1.0471 TD [(by)-513.7(os)14.4(mo)18.1(ti)16.5(c)-524.7(p)0(u)21.2(m)0(p)-516.8(a)0(d)15.1(m)0(i)15.7(n)0(i)18.9(s)0(t)17.7(r)0(a)19.2(t)0(i)16.5(o)0(n)-519.4(w)12.4(er)13.5(e)-524.7(n)0(o)21.2(t)-526.7(af)30.6(fe)13.5(ct)18.4(ed)-514.1(by)]TJ 0 -1.0528 TD [(co)15.1(in)18.9(ci)18.4(de)15.1(nt)-197.4(vi)13.2(ra)19.2(l)-208(i)0(n)13.2(f)0(e)19.2(c)0(t)18.4(i)0(o)18.9(n)0(.)-206(S)11.4(im)15.7(il)16.5(ar)19.2(ly)81.4(,)-210.9(t)0(h)18.9(e)-211.7(le)18.4(ve)15.1(ls)-204.3(of)-202.3(i)13.9(n)0(f)13.9(e)0(c)20.4(t)0(i)16.5(o)0(u)15.5(s)]TJ 0 -1.0471 TD [(vi)18.9(ru)13.9(s)-320.6(i)0(n)-316.9(t)0(h)18.9(e)-325.5(lu)18.8(ng)15.5(s)-326.3(o)0(f)-316.1(p)0(l)18.9(a)0(c)20.4(e)0(b)15.1(o)0(-)19.6(t)0(r)17.3(e)0(a)14.7(t)0(e)18.4(d)-325.1(mi)15.7(ce)-315.3(\(7)19.6(.4)15.9(6\213)16.7(0.)21.6(32)-314.5(lo)13.2(g)]TJ 0 -1.0527 TD [(EI)15.5(D)]TJ 7.472 0 0 6.641 74.438 246.274 Tm (50)Tj 9.963 0 0 9.963 83.906 247.918 Tm [(pe)15.1(r)-218.6(l)0(u)18.8(n)0(g)21.2(\))-218.6(we)16.9(re)-208.4(no)15.5(t)-213.7(s)0(i)17.7(g)0(n)15.5(i)0(f)17.3(i)0(c)18.4(a)0(n)20.8(t)0(l)16.5(y)-217(di)18.9(f)14.7(f)0(e)19.2(r)0(e)19.2(n)0(t)-208.8(f)0(r)18(o)0(m)-209.5(l)0(e)18.4(v)0(e)15.1(l)0(s)]TJ -2.5721 -1.0528 TD [(me)17.7(as)14(ur)19.6(ed)-588.1(in)-584.3(CG)12.6(S)-597.5(2)0(6)21.2(3)0(0)15.5(3)0(-)19.6(t)0(r)17.3(e)0(a)14.7(t)0(e)18.4(d)-592.6(mi)15.7(ce)-588.5(\(6)13.9(.9)21.6(3\213)16.7(0.)15.9(19)-582(lo)13.2(g)]TJ 0 -1.0471 TD [(EI)15.5(D)]TJ 7.472 0 0 6.641 74.438 225.354 Tm (50)Tj 9.963 0 0 9.963 84.302 226.998 Tm [(pe)15.1(r)-252.7(l)0(u)13.2(n)0(g)21.2(;)]TJ 6.974 0 0 6.974 121.72 226.998 Tm 0.0001 Tc (NS)Tj 9.963 0 0 9.963 130.507 226.998 Tm 0 Tc [(\).)-241.7(Th)17.1(us)14.4(,)-250.8(p)0(r)13.9(e)0(s)19.6(e)0(n)20.8(c)0(e)-247(o)0(f)-247.8(C)0(G)12.6(S)-250.4(26)15.5(30)15.5(3)-251.2(h)0(a)15.1(d)-251.2(no)]TJ -7.2498 -1.0528 TD [(si)17.7(gn)15.5(if)17.3(ic)18.4(an)20.8(t)-401.5(e)0(f)30.6(f)0(e)19.2(c)0(t)-391.3(o)0(n)-388.5(t)13.9(he)-388.9(le)18.4(ve)15.1(ls)-386.4(of)-390.1(in)18.9(fe)19.2(ct)12.8(i)13.9(o)0(u)15.5(s)-394.6(in)18.9(fl)11.6(ue)20.8(nz)15.1(a)-393.8(A)]TJ 0 -1.0471 TD [(vi)18.9(ru)13.9(s)-320.6(p)0(r)14(e)0(s)19.6(e)0(n)15.1(t)-321.8(in)-316.9(th)18.9(e)-325.5(l)0(u)18.9(n)0(g)21.2(s)-326.3(4)-325.1(d)0(a)20.8(y)0(s)-315.7(p)0(o)15.5(s)0(t)17.7(-)0(i)17.3(n)0(o)15.5(c)0(u)20.8(l)0(a)18.4(t)0(i)16.5(o)0(n)15.5(.)]TJ /F4 1 Tf 0 -2.39 TD [(Immuno)-8.3(r)32.2(e)0(a)-7.6(ctive)-334.2(e)-6.9(ndothelin)-338.8(conten)-7.8(t)]TJ /F3 1 Tf 1.0015 -1.7527 TD [(Ir)29.4(-E)15.5(T)-238.2(c)0(o)15.1(n)0(t)18.9(e)0(n)15.1(t)-299.1(in)-294.1(th)18.9(e)-302.8(l)0(u)18.9(n)0(g)21.2(s)-303.6(of)-293.3(vi)18.9(ru)19.6(s-)12.8(in)18.9(fe)19.2(ct)18.4(ed)-292.2(mi)15.7(ce)-292.6(\(2)13.9(02)21.2(\213)]TJ -1.0015 -1.0471 TD [(39)-485.2(pg)]TJ /F8 1 Tf 2.4697 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD [(lu)18.9(ng)]TJ 7.472 0 0 6.641 102.841 158.797 Tm (-1)Tj 9.963 0 0 9.963 108.85 154.375 Tm [(\))-491.7(w)0(a)11.2(s)-497(21)21.2(0%)-487.6(hi)13.2(gh)21.2(er)-487.2(th)13.2(an)-485.6(th)18.9(at)-488(me)17.7(as)13.9(ur)19.6(ed)-491.3(in)]TJ -5.076 -1.0528 TD [(sh)20(am)12(-i)17.3(nf)19.6(ec)14.7(te)18.4(d)-501.5(m)0(i)15.7(c)0(e)-491.7(\()0(6)19.6(6)0(\213)16.7(1)0(8)-490.9(p)0(g)]TJ /F8 1 Tf 12.6785 0.2049 TD (.)Tj /F3 1 Tf 0.2731 -0.2049 TD [(lu)18.9(ng)]TJ 7.472 0 0 6.641 204.548 148.309 Tm (-1)Tj 9.963 0 0 9.963 210.557 143.887 Tm 0.4955 Tc (,p)Tj /F9 1 Tf 1.2405 0 TD 0 Tc (<)Tj /F3 1 Tf 0.5406 0 TD [(0.)15.9(05)21.2(\))-503.1(\()0(f)18(i)0(g)13.2(.)-495.5(1\).)]TJ -17.066 -1.0471 TD [(However)18.5(,)-506.8(pretreatm)-13.5(ent)-511.5(with)-512.5(CGS)-516.6(26303,)-510.7(signi)-9.8(ficantly)]TJ 0 -1.0528 TD [(attenu)-10.1(ated)-501.3(this)-504.1(virus-ind)-10.3(uced)-504.6(increase)-508(in)-499(the)-505.8(levels)-506.4(of)]TJ 0 -1.0471 TD [(ir-ET)-427.3(\(p)]TJ /F9 1 Tf 3.3745 0 TD (<)Tj /F3 1 Tf 0.5577 0 TD [(0.05,)-435.5(2-way)-436.5(AN)-9.4(OV)121.5(A\),)-434.2(such)-437.1(that)-440.7(ir-)9.2(ET)-436.6(level)-9.4(s)]TJ 21.2656 43.8571 TD [(wer)-8.2(e)-456.4(only)-460.7(75%)-459.9(higher)-464.2(in)-453.5(virus)-9(-infected)-465.6(mic)-8.2(e)-456.4(\(109\21314)]TJ 0 -1.0471 TD (pg)Tj /F8 1 Tf 1.0015 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (lung)Tj 7.472 0 0 6.641 339.704 543.458 Tm (-1)Tj 9.963 0 0 9.963 345.883 539.036 Tm [(\))-321(than)-330.2(in)-328.3(sham-inf)-10.7(ected)-326.1(mic)-8.2(e)-325.5(\(62\21313)-329.4(pg)]TJ /F8 1 Tf 16.1441 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (lung)Tj 7.472 0 0 6.641 527.131 543.458 Tm (-1)Tj 9.963 0 0 9.963 533.253 539.036 Tm (\))Tj -22.4777 -1.0528 TD [(\(fig)-8.6(.)-330.4(1\).)]TJ /F4 1 Tf 0 -2.5607 TD [(Lu)-6.4(ng)-331.6(wet)-339.8(weight)-8.8(s)]TJ /F3 1 Tf 1.0015 -1.747 TD [(On)-460.7(av)15.1(er)19.2(ag)15.1(e,)-456.8(lu)18.9(ng)-456.8(we)16.9(t)-469.8(w)0(e)16.9(i)0(g)18.9(h)0(t)-459.2(w)0(a)16.9(s)-468.6(45)15.5(\213)11.8(5)0(%)-459.1(g)0(r)13.9(e)0(a)20.4(t)0(e)18.5(r)-469(in)]TJ -1.0015 -1.0527 TD [(vi)13.2(ru)19.6(s-)18.4(in)18.9(fe)13.5(ct)18.4(ed)-531.2(mi)15.7(ce)-525.9(\(1)13.9(60)21.2(\2135)-529.6(mg)12.4(\))-537.3(t)0(h)18.8(a)0(n)-531.2(i)0(n)-527.4(s)0(h)14.3(a)0(m)17.7(-)0(i)17.3(n)0(f)19.6(e)0(c)14.7(t)0(e)18.4(d)]TJ 0 -1.0471 TD [(mi)15.7(ce)-520.2(\(1)48.1(10)15.5(\213)11.8(3)-530(mg)12.4(;)-526.7(p)]TJ /F9 1 Tf 8.2797 0 TD (<)Tj /F3 1 Tf 0.5463 0 TD [(0.)21.6(05)15.5(\))-531.6(\()0(f)18(i)0(g)18.9(.)-529.6(2\).)-527(How)-10.2(ever)28.7(,)-541(i)0(n)-538.8(CGS)]TJ -8.826 -1.0527 TD [(263)-8(03-treated)-426.5(mic)-8.2(e,)-417(lung)-426.5(wet)-418.7(wei)-9(ght)-420.1(was)-423.2(increa)-9.8(sed)-419.3(by)]TJ 0 -1.0471 TD [(only)-381(17\213)-6.8(5%)-379.5(durin)-7.8(g)-376.3(viral)-380.5(infec)-8.6(tion)-377.7(\(135\213)-9.9(6)-376.3(m)0(g)-380.3(i)0(n)-379.5(virus-)]TJ 0 -1.0528 TD [(infec)-8.6(ted)-335.1(lungs)]TJ /F4 1 Tf 6.0092 0 TD (versus)Tj /F3 1 Tf 2.868 0 TD [(1)27.7(16\2132)-331.9(mg)-334.7(in)-339.6(sham-inf)-10.7(ected)-337.5(lungs;)]TJ -8.8773 -1.0471 TD [(fig.)-342.4(2\))-333.2(\(p)]TJ /F9 1 Tf 3.6647 0 TD (=)Tj /F3 1 Tf 0.552 0 TD [(0.001,)-339.6(compa)-7.8(red)-334.3(with)-336.1(p)-6.5(lacebo)-338.2(treatmen)-11.1(t\).)]TJ /F4 1 Tf -4.2167 -2.5607 TD [(End)-6.4(othelin)]TJ 7.472 0 0 6.641 352.8 395.66 Tm (B)Tj 9.963 0 0 9.963 360.624 397.361 Tm [(r)26.5(eceptor)-306(densi)-7.8(ties)]TJ /F3 1 Tf -4.1484 -1.7527 TD [(Th)17.1(e)-507.6(l)0(e)18.4(v)0(e)15.1(l)0(s)-494.5(o)0(f)-498.2(s)0(p)20(e)0(c)14.7(i)0(f)17.3(i)0(c)]TJ 7.472 0 0 6.641 415.049 384.321 Tm [(12)21.3(5)]TJ 9.963 0 0 9.963 425.99 379.899 Tm [(I-)18(ET)98.4(-1)-492.5(bi)13.2(nd)21.2(in)18.8(g)-507.2(o)0(n)-496.6(t)0(r)17.3(a)0(c)14.7(h)0(e)20.8(a)0(l)]TJ -11.7112 -1.0471 TD [(sm)16.9(oo)15.5(th)-425(mu)18.1(sc)13.9(le)-425.4(w)12.4(e)0(r)13.5(e)-433.7(si)17.7(mi)15.7(la)18.4(r)-434.8(i)0(n)-425(a)0(l)18.4(l)-435.6(fo)19.6(ur)-418.5(gr)19.6(ou)21.2(ps)-429.5(of)-424.2(mi)15.7(ce)]TJ 0 -1.0527 TD [(\(t)17.3(wo)11.6(-w)21.4(ay)-440.1(AN)19.1(OV)138.6(A,)-437.6(ta)18.4(bl)18.9(e)-445(1\),)-447.4(alth)-8.2(ough)-458.3(the)-454.6(relat)-11(ive)-454.6(pro-)]TJ 0 -1.0471 TD [(porti)-10.2(ons)-549.8(of)-549.4(ET)]TJ 7.472 0 0 6.641 372.416 346.847 Tm (A)Tj 9.963 0 0 9.963 383.244 348.548 Tm [(and)-549(ET)]TJ 7.472 0 0 6.641 415.105 346.847 Tm (B)Tj 9.963 0 0 9.963 425.537 348.548 Tm [(recept)-9.8(ors)-551.4(differed)-549(between)]TJ ET 0 0 0 1 K 0 J 0 j 0.5 w 2.613 M []0 d 1 i 372.044 776.38 m 372.044 652.881 l S BT /F16 1 Tf 10 0 0 10 347.794 771.157 Tm 0 0 0 1 k (300)Tj ET 372.044 776.13 m 366.544 776.13 l 372.044 754.881 m 366.544 754.881 l 372.044 734.381 m 366.544 734.381 l 372.044 713.631 m 366.544 713.631 l 372.044 693.448 m 366.544 693.448 l 372.044 673.265 m 366.544 673.265 l 372.044 652.836 m 366.544 652.836 l S BT 10 0 0 10 356.794 649.533 Tm (0)Tj -0.5 2.0313 TD (50)Tj -0.425 1.9438 TD (100)Tj 0 2.0251 TD (150)Tj 0.025 2.1751 TD (200)Tj 0 1.9501 TD (250)Tj 8.3595 -13.7505 TD [(T)29.8(reatment)]TJ 0 10 -10 0 325.116 687.714 Tm [(ir-ET)-441.8(pg)-228(lung)]TJ 0 8 -8 0 321.32 737.048 Tm (-1)Tj ET 0.527 w 368.481 652.856 m 531.543 652.856 l S BT 10 0 0 10 404.411 628.158 Tm (Sham)Tj ET 0.498 w 391.268 679.124 m 415.757 679.124 l 415.757 652.881 l 391.268 652.881 l 391.268 679.124 l 403.497 679.181 m 403.497 686.834 l 408.824 686.901 m 398.17 686.901 l S q 415.757 677.995 24.499 -25.113 re W* n 0.36 w 450.086 668.259 m 425.209 643.381 l 447.286 671.059 m 422.408 646.181 l 444.486 673.859 m 419.608 648.981 l 441.686 676.66 m 416.808 651.782 l 438.886 679.46 m 414.008 654.582 l 436.086 682.26 m 411.208 657.382 l 433.286 685.06 m 408.408 660.182 l 430.486 687.86 m 405.608 662.982 l 427.685 690.66 m 402.807 665.782 l S Q 415.757 677.995 24.499 -25.113 re 428.654 678.12 m 428.654 683.515 l 434.228 683.575 m 423.079 683.575 l S 0.5 w 415.757 652.903 m 415.757 648.506 l S BT 10 0 0 10 474.204 628.283 Tm [(V)14.2(irus)]TJ ET 0.498 w 478.566 751.876 m 467.912 751.876 l 473.239 751.809 m 473.239 735.592 l 503.415 703.492 m 492.267 703.492 l 497.605 703.618 m 497.605 697.721 l S BT /F18 1 Tf 10.036 0 0 10.036 482.901 763.519 Tm (*)Tj ET 0.5 w 473.475 759.881 m 473.475 763.631 l 497.6 763.631 l 497.6 759.881 l 484.965 652.944 m 484.965 648.506 l S 0.498 w 460.543 735.622 m 484.965 735.622 l 484.965 652.885 l 460.543 652.885 l 460.543 735.622 l S q 485.027 697.391 24.323 -44.698 re W* n 0.36 w 530.465 677.835 m 494.4 641.77 l 527.665 680.635 m 491.6 644.571 l 524.865 683.435 m 488.8 647.371 l 522.065 686.236 m 486 650.171 l 519.264 689.036 m 483.2 652.971 l 516.464 691.836 m 480.4 655.771 l 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[(during)-416.7(coincident)-409.9(administratio)8.7(n)-417.6(o)0(f)-415.7(CGS)-418.4(26303)-409.2(\()]TJ /F12 1 Tf 20.4716 0 TD (h)Tj /F3 1 Tf 0.8322 0 TD [(\))-417.8(o)0(r)-422.8(placebo)-411.4(\()]TJ /F13 1 Tf 5.7972 0 TD (u)Tj /F14 1 Tf 0.7967 0 TD (\).)Tj -27.8977 -1.0599 TD [(Data)-327.4(are)-335.3(presented)-318.6(as)-331.3(mean\213)]TJ 5.579 0 0 5.579 413.348 575.206 Tm (SEM)Tj 7.97 0 0 7.97 428.315 575.206 Tm (\(n)Tj /F9 1 Tf 0.882 0 TD (=)Tj /F14 1 Tf 0.5548 0 TD [(6\2617\).)-322.5(*:)-332.5(p)]TJ /F9 1 Tf 4.0829 0 TD (<)Tj /F14 1 Tf 0.5477 0 TD (0.05.)Tj ET 0.5 w 369.234 329.54 m 369.234 190.529 l 369.234 190.529 m 533.733 190.529 l 384.984 267.915 m 412.234 267.915 l 412.234 190.541 l 384.984 190.541 l 384.984 267.915 l 401.484 267.915 m 401.484 271.142 l 394.984 271.415 m 408.484 271.415 l 426.901 271.415 m 426.901 274.726 l 420.311 274.915 m 433.686 274.915 l S BT /F20 1 Tf 0 10 -10 0 319.234 228.666 Tm 0 0 0 1 k [(Lung)-228(wet)-228(weight)-456(g)]TJ 10 0 0 10 338.234 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[(sensi)-9(tization)-392(in)-385.2(airway)-388.9(prepar)-8.6(atio)-8.2(ns)-384([7,)-384.8(9,)-382.4(22].)-385.5(Wheth)-7.8(er)]TJ 0 -1.0528 TD [(this)-561(CGS)-556.4(26303-i)-9.3(nduced)-557.4(attenuati)-11.9(on)-553.5(of)-555.1(virus-ind)-10.3(uced)]TJ 0 -1.0471 TD [(loss)-349.3(of)-338.9(ET)]TJ 7.472 0 0 6.641 351.779 435.175 Tm (B)Tj 9.963 0 0 9.963 360.17 436.875 Tm [(receptor)-348.6(densi)-7.8(ty)-339.7(is)-340.8(trans)-9.4(lated)-345.1(into)-343.6(preser)-9.8(ved)]TJ -5.1044 -1.0527 TD (ET)Tj 7.472 0 0 6.641 321.392 424.743 Tm (B)Tj 9.963 0 0 9.963 330.35 426.387 Tm [(recept)-9.8(or-mediated)-402(contracti)-11.1(le)-397(respon)-7.8(ses)-403.4(in)-402.2(tracheal)]TJ -2.1112 -1.0471 TD [(smoo)-7.4(th)-334(musc)-7.8(le)-334.4(must)-339.8(await)-337.7(a)-6.9(dditional)-341.1(studies.)]TJ 1.0015 -1.0527 TD [(An)-529(al)18.5(te)18.4(rn)19.6(at)12.8(e,)-519.4(or)-521(pe)15.1(rh)19.6(ap)15.1(s)-531.2(a)0(d)20.8(d)0(i)13.2(t)0(i)16.5(o)0(n)21.2(a)0(l)18.5(,)-535.3(me)17.7(ch)20.8(an)15.1(is)17.7(m)-533.1(t)0(h)18.9(a)0(t)]TJ -1.0015 -1.0471 TD [(sh)14.4(ou)21.2(ld)-203.1(be)-201.1(co)20.8(ns)20(id)13.2(er)19.2(ed)-201.1(is)-198.6(th)18.9(at)-203.5(th)18.9(e)-206(i)0(n)13.2(c)0(r)19.2(e)0(a)20.4(s)0(e)14(d)-205.6(pr)13.9(od)21.2(uc)15.1(ti)16.5(on)-195(of)-202.3(ET)]TJ 0 -1.0528 TD [(du)15.5(ri)17.3(ng)-365.7(vi)18.9(ra)19.2(l)-373(i)0(n)18.9(f)0(e)13.5(c)0(t)18.4(i)0(o)18.8(n)-370.7(pr)13.9(om)18.1(ot)18.9(ed)-366.1(t)13.9(h)0(e)-366.1(r)0(e)19.2(l)0(e)12.8(a)0(s)19.6(e)-371.1(of)-361.6(in)13.2(fl)17.3(am)17.7(ma)17.7(-)]TJ T* [(to)13.2(ry)-304.7(ce)20.4(ll)-307.9(cy)20.8(to)13.2(ki)18.9(ne)20.8(s)-314.9(a)0(n)15.1(d)-313.7(me)17.7(di)18.9(at)12.8(or)19.6(s,)-303.9(wh)17.3(ic)18.4(h)-313.8(i)0(n)-305.5(t)0(u)13.2(r)0(n)-304.7(c)0(a)20.4(u)0(s)14.3(e)0(d)-303.5(a)]TJ 0 -1.0471 TD [(re)13.5(du)21.2(ct)12.8(i)13.9(o)0(n)-462.5(i)0(n)-464.8(E)12.2(T)]TJ 7.472 0 0 6.641 374.683 361.984 Tm (B)Tj 9.963 0 0 9.963 384.321 363.628 Tm [(re)13.5(ce)20.4(pt)13.2(or)-458.4(de)15.1(ns)20(it)16.5(y)73.2(.)-472.7(C)11.6(o)0(n)15.5(s)0(i)17.7(s)0(t)17.7(e)0(n)15.1(t)-469.8(wi)14.9(t)13.9(h)-473.1(th)18.9(is)]TJ -7.5286 -1.0527 TD [(la)12.8(tt)22.2(er)-515.7(in)18.9(di)13.2(re)19.2(ct)-516.5(me)17.7(ch)20.8(an)15.1(is)17.7(m,)-516.4(E)12.2(T)91.9(-1)-515.3(ha)15.1(s)-519.8(d)0(e)15.1(m)0(o)18.1(n)0(s)20(t)0(r)17.3(a)0(b)15.1(l)0(e)-516.5(p)0(r)19.6(o)0(-)]TJ 0 -1.0471 TD [(in)13.2(fl)17.3(am)17.7(ma)17.7(to)18.8(ry)-213.7(ef)24.9(fe)19.2(ct)18.4(s)-223.9(i)0(n)-208.8(t)0(h)13.2(e)-217.4(ai)12.8(rw)21.4(ay)15.1(s,)-212.9(in)18.9(cl)18.5(ud)15.5(in)18.9(g)-222.7(t)0(h)18.9(e)-223.1(in)18.9(fl)17.3(ux)-212.1(of)]TJ 0 -1.0527 TD [(eo)15.1(si)17.7(no)15.5(ph)21.2(il)16.5(s)-440.1(i)0(n)-425(a)-439.3(mu)18.1(ri)17.3(ne)-428.7(mo)12.4(de)20.8(l)-441.3(o)0(f)-424.2(a)0(l)12.8(l)0(e)18.4(r)20.4(gi)18.9(c)-439.3(i)0(n)18.8(f)0(l)17.3(a)0(m)12(m)0(a)17.7(t)0(i)16.5(o)0(n)]TJ 0 -1.0471 TD [([2)13.9(3].)-236.8(In)-247.8(addition,)-253(vario)-8.2(us)-247.4(inflamm)-12.7(atory)-247.2(cell)-253.3(cytokine)-11.7(s,)-241.3(in-)]TJ 0 -1.0528 TD [(cludi)-9(ng)-547.8(interleuk)-10.7(in)-544.5(\(IL)-9.6(\)-1)-546.1(and)-549(tum)-7.8(our)-544.5(necros)-8.2(is)-551.4(factor)]TJ 0 -1.0471 TD [(\(T)-7.2(NF\)-)]TJ /F9 1 Tf 2.851 0 TD (a)Tj /F3 1 Tf 0.6373 0 TD [(,)-444.2(h)-6.5(ave)-447(been)-453.4(reported)-456.3(to)-447.8(modul)-9.3(ate)-449.3(ET)-413.8(recept)-9.8(or)]TJ -3.4883 -1.0528 TD [(level)-9.4(s)-269.4([24,)-277.4(25].)-271.7(In)-270.6(this)-276.5(regard)-8.6(,)-267.8(i)0(t)-273.7(i)0(s)-272.6(inte)-8.2(resting)-275.1(to)-271.4(note)-279(that)]TJ 0 -1.0471 TD [(CGS)-345.9(26303)-345.2(inhibit)-9.2(ed)-343.4(the)-340.8(virus-ind)-10.3(uced)-345.3(increase)-348.6(in)-339.7(lung)]TJ 0 -1.0528 TD [(wet)-248(weight,)-247.4(perhaps)-247.9(by)-240.5(blunting)-246.6(the)-244.1(pro-i)-9.4(nflamma)-10.7(tory)-240.3(ef-)]TJ T* [(fects)-556.1(of)-549.4(ET)81.3(-1.)-555.5(However)24.2(,)-552.4(the)-551.4(CGS)-550.8(26303-i)-9.3(nduced)-551.7(re-)]TJ 0 -1.0471 TD [(duc)-8.4(tion)-372(in)-373.8(virus)-9(-indu)-7.8(ced)-373(lung)-375.3(weig)-9.8(ht)-373.8(may)-375.7(be)-371.8(other)-8.2(wise)]TJ 0 -1.0528 TD [(exp)-8.4(lained)-220.7(by)-217.8(consi)-7.8(dering)-219.5(haemo)-7.8(dynam)-7.4(ic)-220.6(influences)-10.7(,)-216.6(such)]TJ 0 -1.0471 TD [(as)-338.9(a)-331.2(reduct)-9.4(ion)-334.7(in)-334(ET)81.3(-1-induced)-344.1(venocons)-8.1(trictio)-11.2(n)-330.8(and)-332.7(the)]TJ 0 -1.0528 TD [(associ)-10.1(ated)-336.3(pulm)-8.6(onary)-335.9(congest)-9.7(ion.)]TJ 1.0015 -1.0471 TD [(Th)17.1(e)-319.8(l)0(e)18.4(v)0(e)20.8(l)0(s)-306.7(o)0(f)-310.4(i)0(n)18.9(f)0(e)19.2(c)0(t)12.8(i)0(o)18.9(u)0(s)-304.3(v)0(i)18.9(r)0(u)13.9(s)-314.9(me)17.7(as)19.6(ur)13.9(ed)-303.5(in)-311.2(th)18.8(e)-314.2(l)0(u)18.9(n)0(g)15.5(s)-314.9(of)]TJ -1.0015 -1.0527 TD [(mi)15.7(ce)-366.5(on)-365.7(da)20.8(y)-376.3(f)0(o)19.6(u)0(r)-367.3(p)0(o)21.2(s)0(t)17.7(-)0(i)17.3(n)0(o)15.5(c)0(u)15.1(l)13.9(at)12.8(io)18.9(n)-370.7(w)0(e)16.9(r)0(e)-367.7(n)0(o)15.5(t)-373(si)17.7(gn)21.2(if)17.3(ic)12.8(an)20.8(tl)16.5(y)]TJ 0 -1.0471 TD [(in)13.2(fl)17.3(ue)20.8(nc)15.1(ed)-343.4(by)-343(co)15.1(nc)15.1(om)18.1(it)16.5(an)20.8(t)-356(a)0(d)20.8(m)0(i)15.7(n)0(i)13.2(s)0(t)17.7(r)0(a)19.2(t)0(i)16.5(o)0(n)-343(o)0(f)-344.6(C)0(G)12.6(S)-352.8(26)21.2(30)15.5(3)]TJ 0 -1.0527 TD [(an)15.1(d,)-325.5(si)17.7(mi)21.4(la)12.8(rl)17.3(y)73.2(,)-330.4(th)13.2(e)-331.2(p)0(l)18.8(a)0(s)19.6(m)0(a)-329.4(l)0(e)18.5(v)0(e)20.8(l)0(s)-329.5(o)0(f)-321.8(C)0(G)18.3(S)-335.7(26)21.2(30)15.5(3)-330.8(w)0(e)16.9(r)0(e)-327.9(n)0(o)21.2(t)]TJ 0 -1.0471 TD [(si)17.7(gn)15.5(if)17.3(ic)18.4(an)15.1(tl)16.5(y)-427.6(a)0(f)30.6(f)0(e)13.5(c)0(t)18.4(e)0(d)-417.3(b)0(y)-422.6(r)0(e)19.2(s)0(p)20(i)0(r)17.3(a)0(t)12.8(o)0(r)19.6(y)-427.6(tr)17.3(ac)14.7(t)-424.2(v)0(i)13.2(r)0(a)19.2(l)-429.9(in)18.9(fe)19.2(ct)12.8(i)13.9(o)0(n)15.5(.)]TJ 0 -1.0527 TD [(Th)17.1(us)14.4(,)-267.8(i)0(t)-268.1(i)0(s)-261.2(u)0(n)15.5(l)0(i)16.5(k)0(e)20.8(l)0(y)-265.7(t)0(h)18.9(a)0(t)-266.1(t)0(h)18.9(e)-268.6(ob)15.5(se)19.6(rv)13.9(ed)-263.7(ch)20.8(an)15.1(ge)20.8(s)-275.1(i)0(n)-265.7(t)0(h)18.8(e)-274.3(l)13.9(e)0(v)15.1(e)0(l)18.4(s)]TJ T* [(of)-355.9(ir)28.6(-E)21.2(T)-300.8(a)0(n)20.8(d)-365(E)12.2(T)]TJ 7.472 0 0 6.641 374.286 142.242 Tm (B)Tj 9.963 0 0 9.963 382.847 143.887 Tm [(re)19.2(ce)14.7(pt)18.9(or)-350.3(de)15.1(ns)20(it)16.5(y)-359.3(i)0(n)-351(v)0(i)18.9(r)0(u)19.6(s)0(-)12.8(i)0(n)18.9(f)0(e)19.2(c)0(t)18.5(e)0(d)-354.8(m)0(i)15.7(c)0(e)]TJ -7.3806 -1.0471 TD [(tr)17.3(ea)14.7(te)18.4(d)-421.9(w)0(i)14.9(t)0(h)-407.9(C)0(G)12.6(S)-415.4(26)15.5(30)15.5(3)-416.2(\()0(c)13.5(o)0(m)18.1(p)0(a)20.8(r)0(e)13.5(d)-416.2(wi)14.9(th)-413.6(pl)18.9(ac)14.7(eb)20.8(o\))-412.8(we)16.9(re)]TJ 0 -1.0528 TD [(du)15.5(e)-393.8(t)0(o)-385.2(i)0(m)15.7(p)0(a)15.1(i)0(r)17.3(e)0(d)-383.2(g)0(r)13.9(o)0(w)17.3(t)0(h)-385.2(o)0(f)-384.4(v)0(i)18.8(r)0(u)13.9(s)-394.6(in)-385.2(th)18.9(e)-393.8(a)0(i)12.8(r)0(w)15.7(a)0(y)20.8(s)-394.6(of)-384.4(CG)12.6(S)]TJ 0 -1.0471 TD [(26)15.5(30)15.5(3-)19.6(tr)17.3(ea)20.4(te)12.8(d)-512.9(m)0(i)15.7(c)0(e)20.4(.)-512.5(Si)13.9(mi)15.7(la)18.4(rl)17.3(y)73.2(,)-512.5(th)18.9(e)-519(d)0(i)18.9(f)20.4(fe)19.2(re)13.5(nt)18.9(ia)18.4(l)-515.3(e)0(f)30.6(f)0(e)13.5(c)0(t)-505.1(o)0(f)]TJ ET 1 i 58.28 749.367 227.168 -0.51 re f 58.28 734.4 81.468 -0.283 re f 147.912 734.4 137.537 -0.283 re f 58.28 717.959 227.168 -0.454 re f 58.28 673.171 227.168 -0.51 re f BT /F2 1 Tf 8.966 0 0 8.966 58.28 772.667 Tm [(Table)-331.9(1.)-336.7(\261)-333.2(Influence)-727.3(of)-728.7(CGS)-730.8(26303)-723.8(on)-729.6(virus-induced)]TJ 0 -1.0559 TD [(changes)-520.5(in)-526.2(the)-524.5(densities)-526.2(of)-520.1(endothelin)-527.7(\(ET\))]TJ 6.725 0 0 5.977 240.718 761.669 Tm (A)Tj 8.966 0 0 8.966 249.902 763.2 Tm [(and)-525.4(ET)]TJ 6.725 0 0 5.977 280.913 761.669 Tm (B)Tj 8.966 0 0 8.966 58.28 753.732 Tm [(receptors)-334.1(on)-331.3(murine)-336.3(tracheal)-335.8(smooth)-327.6(muscle)]TJ /F3 1 Tf 2.3332 -1.7072 TD [(T)34.9(reatment)-7350.1(ET)-303.1(receptor)-334.7(density)]TJ -2.3332 -1.6692 TD [(CGS)-333.9(26303)-1856.4(V)47.8(irus)-2468.4(T)66.6(otal)-3562.3(ET)]TJ 6.725 0 0 5.977 219.402 721.984 Tm (A)Tj 8.966 0 0 8.966 257.556 723.458 Tm (ET)Tj 6.725 0 0 5.977 268.384 721.984 Tm (B)Tj 8.966 0 0 8.966 58.28 705.543 Tm [(No)-5870(No)-2341.8(95.8\2137.6)-1871.3(28.2\2136.2)-1877.6(67.6\21312)]TJ 0 -1.0559 TD [(Y)92(e)0(s)-5640(N)0(o)-2341.8(91.1\2137.9)-1871.3(34.8\2135.2)-1877.6(56.3\2134.7)]TJ T* [(No)-5756.1(Y)92(e)0(s)-2225.6(99.7\2138.7)-1871.3(68.0\21310)-2127.6(31.7\2135.3)]TJ T* [(Y)92(e)0(s)-5526.2(Y)92(es)-2472.2(105\21310)-2124.7(50.6\2135.7)-1877.6(54.3\2138.0)]TJ 0 -1.6123 TD [(Data)-306.7(are)-308.8(shown)-311.8(as)-303.5(mean\213)]TJ 6.276 0 0 6.276 151.54 662.683 Tm 0.0001 Tc (SEM)Tj 8.966 0 0 8.966 167.131 662.683 Tm 0 Tc (\(n)Tj /F9 1 Tf 0.822 0 TD (=)Tj /F3 1 Tf 0.5501 0 TD [(7\2618\).)-311.7(*:)-304.9(density)-312(of)-303.5(autoradio-)]TJ -13.5121 -1.0559 TD [(graphic)-334.4(grains)-336.4(associated)-336.3(with)-334.8(specific)]TJ 6.725 0 0 5.977 201.146 657.184 Tm 0.0001 Tc (125)Tj 8.966 0 0 8.966 211.238 653.216 Tm 0 Tc [(I-ET)81.6(-1)-335.1(binding.)]TJ ET 0 0 0 1 K 0 J 0 j 0.5 w 2.613 M []0 d 99.315 362.07 m 99.315 216.822 l 99.315 216.822 m 289.063 216.822 l 99.315 216.822 m 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[(el)18.4(ev)15.1(at)18.4(ed)-332(le)12.8(ve)20.8(ls)-335.1(of)-338.9(en)20.8(do)15.5(th)18.9(el)18.4(in)18.9(-1)-338.9(pr)19.6(es)19.6(en)15.1(t)-344.6(i)0(n)-334(v)0(i)18.9(r)0(a)19.2(l)0(l)16.5(y)-347.9(i)13.9(n)0(f)14(e)0(c)20.4(t)0(e)12.8(d)]TJ 0 -1.0527 TD [(mi)15.7(ce)-486(ca)14.7(us)20(ed)-491.3(en)20.8(do)15.5(th)18.9(el)18.4(in)]TJ 7.472 0 0 6.641 154.261 288.794 Tm (B)Tj 9.963 0 0 9.963 164.183 290.438 Tm [(re)19.2(ce)14.7(pt)18.9(or)-486.8(do)15.5(wn)17.3(re)19.2(gu)15.5(la)18.4(ti)16.5(on)15.5(.)-495.5(F)11.4(ur)25.3(-)]TJ -10.6299 -1.0471 TD [(th)18.9(er)13.5(mo)18.1(re)19.2(,)-358.9(t)0(h)18.9(e)-359.7(re)19.2(su)14.4(lt)16.5(s)-354.8(o)0(f)-350.2(t)0(h)18.9(e)-359.7(cu)15.1(rr)18(en)20.8(t)-361.7(s)0(t)17.7(u)0(d)21.2(y)-359.3(us)14.4(in)18.9(g)-359.3(a)0(n)-349.1(e)0(n)20.8(d)0(o)15.5(-)]TJ 0 -1.0527 TD [(th)18.9(el)18.4(in)13.2(-c)19.2(on)15.5(ve)20.8(rt)17.3(in)18.9(g)-547.1(e)0(n)20.8(z)0(y)15.1(m)0(e)-534.3(i)0(n)18.9(h)0(i)13.2(b)0(i)18.9(t)0(o)18.9(r)-543(wi)14.9(ll)-535.5(be)-531.2(of)-538(st)17.7(ra)19.2(te)18.4(gi)13.2(c)]TJ 0 -1.0471 TD [(im)15.7(po)21.2(rt)11.6(an)20.8(ce)-520.2(in)-521.8(fu)13.9(tu)18.9(re)-521.4(st)17.7(ud)15.5(ie)18.4(s)-531.2(a)0(i)18.4(m)0(e)17.7(d)-530(at)-522.2(de)15.1(te)18.4(rm)16.5(in)18.9(in)18.8(g)-530(t)0(h)13.2(e)]TJ 0 -1.0528 TD [(me)17.7(di)18.9(at)12.8(or)-287.7(ro)19.6(le)-288.8(of)-293.3(en)20.8(do)15.5(th)18.9(el)18.4(in)18.9(-1)-287.7(in)-294.1(vi)18.9(ru)19.6(s-)18.4(in)13.2(du)21.2(ce)14.7(d)-296.7(a)0(i)18.5(r)0(w)15.7(a)0(y)-286.5(h)0(y)15.5(-)]TJ 0 -1.0471 TD [(pe)15.1(rr)18(es)19.6(po)15.5(ns)20(iv)18.9(en)15.1(es)19.6(s.)]TJ /F6 1 Tf 8.966 0 0 8.966 151.257 219.118 Tm [(Referenc)8.7(es)]TJ /F3 1 Tf -10.3696 -1.8905 TD [(1.)-1766.5(Springall)-311.7(DR,)-313.4(Howarth)-309.6(PH,)-313.6(Counihan)-312.6(H,)-315.5(Djukanovic)-312.3(R,)]TJ 2.5165 -1.1065 TD [(Holgate)-396.1(ST)74.8(,)-394.9(Polak)-396.5(JM.)-389.5(Endothelin)-397.3(immunoreactivity)-406.1(of)]TJ 0 -1.1128 TD [(airway)-509(epithelium)-518(in)-507.2(asthmatic)-517.1(patients.)]TJ /F4 1 Tf 17.3374 0 TD (Lancet)Tj /F3 1 Tf 3.212 0 TD (1991;)Tj -20.5495 -1.1128 TD [(337:)-329.1(697\261701.)]TJ -2.5165 -1.1128 TD [(2.)-1766.5(Andersson)-305.3(SE,)-307.5(Zackrisson)-307.2(C,)-304.3(Behrens)-306.3(K,)]TJ /F4 1 Tf 19.4809 0 TD [(et)-304.6(al)]TJ /F3 1 Tf 1.7831 0 TD [(.)-306.4(Effec)10.2(t)-311.7(o)0(f)]TJ -18.7474 -1.1065 TD [(aller)9.9(gen)-257.5(provocation)-258.9(on)-258.2(inflammatory)-265.5(cell)-255.2(profile)-262.1(and)-257.5(en-)]TJ 0 -1.1128 TD [(dothelin-like)-606.5(immunoreactivity)-608.5(in)-602.1(guinea-pig)-600.9(airways.)]TJ /F4 1 Tf T* [(Aller)31.3(gy)]TJ /F3 1 Tf 3.2057 0 TD [(1995;)-334.9(50:)-329.7(349\261358.)]TJ -5.7222 -1.1065 TD [(3.)-1766.5(Finsnes)-227.2(F)77.8(,)-230.5(Skjonsberg)-219.1(OH,)-223.5(T)60.2(onnessen)-225.5(T)72.9(,)-230.5(Naess)-230.3(O,)-227(L)53.9(yberg)]TJ 2.5165 -1.1128 TD [(GT)76.4(,)-306.4(Christensen)-316.6(G.)-309.2(Endothelin)-308.8(production)-310.2(and)-308.1(effects)-304.8(of)]TJ 27.9978 73.5671 TD [(endothelin)-507.4(antagonism)-508.1(during)-512.5(experimental)-508.8(airway)-509(in-)]TJ 0 -1.1191 TD (flammation.)Tj /F4 1 Tf 5.3429 0 TD [(Am)-536.9(J)-543(Respir)-542.4(Crit)-539.8(Car)27.2(e)-543.1(Med)]TJ /F3 1 Tf 12.8924 0 TD [(1997;)-543.6(155:)]TJ -18.2353 -1.1128 TD (1404\2611412.)Tj -2.5165 -1.1128 TD [(4.)-1766.5(Carr)-251(MJ,)-250.4(Spalding)-252(LJ,)-253.2(Goldie)-251.4(RG,)-250.2(Henry)-254.4(PJ.)-248.3(Distribution)]TJ 2.5165 -1.1128 TD [(of)-240.2(immunoreactive)-244.1(endothelin)-241.9(in)-241.7(the)-240.9(lungs)-241.3(of)-240.2(mice)-242.1(during)]TJ 0 -1.1192 TD [(respiratory)-281(viral)-281.2(infection.)]TJ /F4 1 Tf 10.6604 0 TD [(Eur)-281.4(Respir)-276.9(J)]TJ /F3 1 Tf 5.3429 0 TD [(1998;)-278(1)32.1(1)0(:)-279.6(79\26185.)]TJ -18.5198 -1.1065 TD [(5.)-1766.5(Goldie)-276.7(RG,)-275.5(Knott)-277.4(PG,)-275.6(Carr)-276.3(MJ,)-275.7(Hay)-279.8(DW)87(,)-274.8(Henry)-279.7(PJ.)-273.5(The)]TJ 2.5165 -1.1192 TD [(endothelins)-413.3(in)-412.4(the)-405.3(pulmonary)-417.7(system.)]TJ /F4 1 Tf 15.9021 0 TD [(Pulm)-411.8(Pharmacol)]TJ /F3 1 Tf -15.9021 -1.1128 TD [(1996;)-334.9(9:)-330.2(69\26193.)]TJ -2.5165 -1.1128 TD [(6.)-1766.5(Behera)-830.2(AK,)-830.5(Kumar)-831.7(M,)-831.4(Matsuse)-829.7(H,)-827.7(Lockey)-828.3(RF)73.6(,)]TJ 2.5165 -1.1192 TD [(Mohapatra)-504.6(SS.)-498.6(Respiratory)-506(syncitial)-498.9(virus)-507.8(induces)-499.1(the)]TJ 0 -1.1128 TD [(expression)-411.2(of)-404.6(5)]TJ /F10 1 Tf 6.3735 0 TD (')Tj /F3 1 Tf 0.2213 0 TD [(lipoxygenase)-403.8(and)-409.2(endothelin-1)-412.5(in)-406.1(bron-)]TJ -6.5948 -1.1192 TD [(chial)-583.5(epithelial)-587.8(cells.)]TJ /F4 1 Tf 9.3769 0 TD [(Biochem)-586.9(Biophys)-578.7(Res)-584.6(Commun)]TJ /F3 1 Tf -9.3769 -1.1128 TD [(1998;)-334.9(251:)-329.1(704\261709.)]TJ -2.5165 -1.1128 TD [(7.)-1766.5(Henry)-216.5(PJ,)-210.3(Goldie)-219.8(RG.)-212.2(ET)]TJ 6.725 0 0 5.977 422.079 630.765 Tm (B)Tj 8.966 0 0 8.966 428.542 632.239 Tm [(but)-209.5(not)-215.8(ET)]TJ 6.725 0 0 5.977 465.505 630.765 Tm (A)Tj 8.966 0 0 8.966 472.252 632.239 Tm -0.0002 Tc (receptor-mediated)Tj -15.6555 -1.1128 TD 0 Tc [(contractions)-577.9(to)-576.8(endothelin-1)-576.9(attenuated)-582.9(by)-574.4(respiratory)]TJ 0 -1.1192 TD [(tract)-407.4(viral)-407.6(infection)-407.7(in)-406(mouse)-405(airways.)]TJ /F4 1 Tf 16.1677 0 TD [(Br)-402(J)-410.3(Pharmacol)]TJ /F3 1 Tf -16.1677 -1.1128 TD [(1994;)-334.9(1)32.1(12:)-329.7(1)25.8(188\2611)34.2(194.)]TJ -2.5165 -1.1128 TD [(8.)-1766.5(Carr)-213(MJ,)-212.4(Goldie)-219.8(RG,)-212.2(Henry)-210.1(PJ.)-216.6(T)34.9(ime)-217.5(course)-213.6(of)-214.9(changes)-211.9(in)]TJ 2.5165 -1.1128 TD [(ET\(B\))-415.7(receptor)-416.9(density)-413.2(and)-415.6(function)-418.7(in)-412.4(tracheal)-418(airway)]TJ 0 -1.1192 TD [(smooth)-603.1(muscle)-608.9(during)-601(viral)-603.6(infection)-610.1(in)-602.1(mice.)]TJ /F4 1 Tf 20.7897 0 TD [(Br)-604.3(J)]TJ -20.7897 -1.1128 TD (Pharmacol)Tj /F3 1 Tf 4.7485 0 TD [(1996;)-334.9(1)32.1(17:)-329.7(1222\2611228.)]TJ -7.265 -1.1128 TD [(9.)-1766.5(Henry)-279.7(PJ.)-279.9(Endothelin-1)-277.1(\(ET)82.1(-1\)-induced)-283.7(contraction)-280(in)-279.6(rat)]TJ 2.5165 -1.1192 TD [(isolated)-345.3(trachea:)-348.5(involvement)-344.4(of)-341.4(ET)]TJ 6.725 0 0 5.977 461.82 540.794 Tm (A)Tj 8.966 0 0 8.966 469.701 542.268 Tm [(and)-346(ET)]TJ 6.725 0 0 5.977 496.516 540.794 Tm (B)Tj 8.966 0 0 8.966 504.057 542.268 Tm (receptors)Tj -19.2027 -1.1128 TD [(and)-225.9(multiple)-234.7(signal)-230.3(transduction)-236.7(systems.)]TJ /F4 1 Tf 16.5154 0 TD [(Br)-231.3(J)-233.2(Pharmacol)]TJ /F3 1 Tf -16.5154 -1.1192 TD [(1993;)-334.9(1)32.1(10:)-329.7(435\261441.)]TJ -2.5165 -1.1065 TD [(10.)-1266.5(Henry)-273.4(PJ,)-273.6(Rigby)-276.4(PJ,)-273.5(Mackenzie)-275.5(JS,)-273.6(Goldie)-276.7(RG.)-275.5(Ef)15.5(fect)-279.1(of)]TJ 2.5165 -1.1192 TD [(respiratory)-597.1(tract)-590.8(viral)-597.3(infection)-591.1(on)-593.3(murine)-590(airway)]TJ /F9 1 Tf 21.9468 0 TD (b)Tj /F3 1 Tf 0.5501 0 TD (-)Tj -22.4969 -1.1128 TD [(adrenoceptor)-604.3(function,)-611.3(distribution)-608.6(and)-605.3(density)61.1(.)]TJ /F4 1 Tf 20.7897 0 TD [(Br)-604.3(J)]TJ -20.7897 -1.1192 TD (Pharmacol)Tj /F3 1 Tf 4.7485 0 TD [(1991;)-334.9(104:)-329.1(914\261921.)]TJ -7.265 -1.1128 TD [(1)25.8(1)0(.)-1292.3(Thomson)-262.2(WR.)-271.4(Use)-262(of)-265.5(moving)-266.8(averages)-269.1(and)-263.8(interpolation)]TJ 2.5165 -1.1128 TD [(to)-431.3(estimate)-441.7(median-effec)6.4(tive)-438.8(dose.)]TJ /F4 1 Tf 14.5237 0 TD [(Bacteriol)-436.7(Rev)]TJ /F3 1 Tf 6.0257 0 TD (1947;)Tj -20.5495 -1.1128 TD [(1)32.1(1)0(:)-336.5(1)32.1(15\261145.)]TJ -2.5165 -1.1128 TD [(12.)-1266.5(Henry)-463.1(PJ,)-463.2(Rigby)-459.8(PJ,)-463.2(Self)-467.6(GJ,)-461.6(Preuss)-460.2(JM,)-465.3(Goldie)-460(RG.)]TJ 2.5165 -1.1192 TD [(Relationship)-331.3(between)-330.2(endothelin-1)-330.3(binding)-330(site)-326.2(densities)]TJ 0 -1.1128 TD [(and)-472.5(constrictor)-484.7(activities)-475.2(in)-475.6(human)-479.6(and)-472.5(animal)-477.3(airway)]TJ 0 -1.1192 TD [(smooth)-331.2(muscle.)]TJ /F4 1 Tf 6.6454 0 TD [(Br)-332.5(J)-334.4(Pharmacol)]TJ /F3 1 Tf 6.8414 0 TD [(1990;)-334.9(100:)-329.1(786\261792.)]TJ -16.0033 -1.1065 TD [(13.)-1266.5(W)70.8(ong)-434.8(M,)-433(Jeng)-441.5(A)85.7(Y)123.6(.)-432.9(Determination)-444.6(of)-436.2(tissue)-440.2(endothelin)]TJ 2.5165 -1.1192 TD (levels.)Tj /F4 1 Tf 2.8832 0 TD [(Biochem)-333.9(Mol)-336.4(Biol)-335(Int)]TJ /F3 1 Tf 9.1303 0 TD [(1995;)-334.9(35:)-336(1359\2611364.)]TJ -14.5301 -1.1128 TD [(14.)-1266.5(Y)92(ang)-213.2(Q,)-220.7(Battistini)-223.5(B,)-215.8(D)]TJ /F10 1 Tf 11.6594 0 TD (')Tj /F3 1 Tf 0.2213 0 TD [(Orleans-Juste)-221.2(P)103.1(,)-217.9(Jeng)-213.9(A)79.4(Y)123.6(,)-211.6(Sirois)-223.8(P)103.1(.)]TJ -9.3642 -1.1128 TD [(Modulation)-343.9(of)-341.4(endothelin)-349.4(production)-341.8(and)-339.7(metabolism)-348(in)]TJ 0 -1.1192 TD [(guinea-pig)-442.8(tracheal)-437(epithelial)-442.4(cells)-439.3(by)-441.6(peptidase)-437(inhibi-)]TJ 0 -1.1128 TD (tors.)Tj /F4 1 Tf 2.0233 0 TD [(Am)-296.6(J)-290.1(Respir)-295.8(Crit)-299.5(Car)33.6(e)-296.5(Med)]TJ /F3 1 Tf 11.4192 0 TD [(1997;)-290.7(155:)-297.5(1884\2611889.)]TJ -15.959 -1.1128 TD [(15.)-1266.5(Y)92(oshimura)-470(H,)-473.6(Nishimura)-475.2(J,)-471.5(Sakihara)-477.7(C,)-475(Kobayashi)-469.7(S,)]TJ 2.5165 -1.1128 TD [(T)60.2(akahashi)-315(S,)-310.8(Kanaide)-317.6(H.)-315.5(Expression)-313.7(and)-314.4(function)-317.6(of)-316.1(en-)]TJ 0 -1.1192 TD [(dothelins,)-398.4(endothelin)-393.6(receptors,)-395.2(and)-396.6(endothelin)-399.9(convert-)]TJ 0 -1.1128 TD [(ing)-285.4(enzyme)-282.7(in)-285.9(the)-285.2(porcine)-290.2(trachea.)]TJ /F4 1 Tf 14.1001 0 TD [(Am)-284(J)-283.8(Respir)-289.5(Cell)-285.4(Mol)]TJ -14.1001 -1.1192 TD (Biol)Tj /F3 1 Tf 1.9791 0 TD [(1997;)-334.9(17:)-329.7(471\261480.)]TJ -4.4956 -1.1065 TD [(16.)-1266.5(Hennet)-451.1(T)72.9(,)-451.8(Ziltener)-456(HJ,)-449(Frei)-448.6(K,)-454.6(Peterhans)-453.1(E.)-448.5(A)-451.7(kinetic)]TJ 2.5165 -1.1192 TD [(study)-291.9(of)-297.1(immune)-293.7(mediators)-300.9(in)-292.2(the)-297.8(lungs)-291.9(of)-297.1(mice)-298.9(infected)]TJ 0 -1.1128 TD [(with)-334.8(influenza)-335.6(A)-262.1(virus.)]TJ /F4 1 Tf 9.6424 0 TD [(J)-334.4(Immunol)]TJ /F3 1 Tf 4.6347 0 TD [(1992;)-334.9(149:)-329.1(932\261939.)]TJ -16.7937 -1.1128 TD [(17.)-1266.5(Nakano)-347.5(J,)-345(T)66.6(akizawa)-349(H,)-347.1(Ohtoshi)-347.1(T)72.9(,)]TJ /F4 1 Tf 16.9897 0 TD [(et)-348.9(al)]TJ /F3 1 Tf 1.8273 0 TD [(.)-344.4(Endotoxin)-345.1(and)]TJ -16.3005 -1.1192 TD [(pro-inflammatory)-126.3(cytokines)-121.1(stimulate)-121.4(endothelin-1)-121.7(expres-)]TJ 0 -1.1128 TD [(sion)-545.3(and)-542(release)-543.8(by)-542.8(airway)-546.9(epithelial)-549.9(cells.)]TJ /F4 1 Tf 19.0256 0 TD [(Clin)-544.9(Exp)]TJ -19.0256 -1.1192 TD [(Aller)31.3(gy)]TJ /F3 1 Tf 3.2057 0 TD [(1994;)-334.9(24:)-336(330\261336.)]TJ -5.7222 -1.1065 TD [(18.)-1266.5(Saleh)-339.4(D,)-347.1(Furukawa)-339.7(K,)-340.8(T)60.2(sao)-340.9(MS,)]TJ /F4 1 Tf 16.2183 0 TD [(et)-342.6(al)]TJ /F3 1 Tf 1.821 0 TD [(.)-344.4(Elevated)-338.6(expres-)]TJ -15.5227 -1.1192 TD [(sion)-248.1(of)-246.5(endothelin-1)-254.5(and)-251.2(endothelin-converting)-248.8(enzyme-1)]TJ 0 -1.1128 TD [(in)-254.3(idiopathic)-263.2(pulmonary)-253.3(fibrosis:)-259.8(possible)-262.1(involvement)-255.9(of)]TJ 0 -1.1192 TD [(proinflammatory)-410.3(cytokines.)]TJ /F4 1 Tf 11.5583 0 TD [(Am)-410.4(J)-404(Respir)-409.6(Cell)-405.6(Mol)-412.3(Biol)]TJ /F3 1 Tf -11.5583 -1.1128 TD [(1997;)-334.9(16:)-329.6(187\261193.)]TJ 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