PT -期刊文章盟Kunitake R盟——Kuwano K盟——宫崎骏,H盟——Hagimoto N野本盟——Y盟——Hara N TI -细胞凋亡过程中肺结节病肉芽肿性炎症的DP - 1999年6月01 TA -欧洲呼吸杂志》第六PG - 1329 - 1337 - 13 IP - 6 4099 - //www.qdcxjkg.com/content/13/6/1329.short 4100 - //www.qdcxjkg.com/content/13/6/1329.full所以欧元和J1999 6月01;AB -结节病是一种病因不明的慢性炎症性疾病，其特征是形成非坏死性肉芽肿。病程通常自限性，肉芽肿自然消退。在免疫系统中，Fas抗原(Fas)和Fas配体(FasL)通过诱导细胞凋亡参与免疫反应的下调。因此，我们推测Fas/FasL通路和细胞凋亡可能与结节病肉芽肿炎症的发生过程有关。采用末端脱氧核苷酸转移酶介导的生物素缺口末端标记(TUNEL)来评估脱氧核糖核酸链断裂作为细胞凋亡的一个特征。免疫组化检测Fas和FasL蛋白，逆转录聚合酶链反应(RT-PCR)和RT原位PCR检测FasL信使核糖核酸(mRNA)。结节病患者肉芽肿内上皮样组织细胞、淋巴细胞及支气管肺泡灌洗淋巴细胞均检测到TUNEL阳性信号。在这些细胞中也检测到Fas阳性信号。FasL mRNA在20例结节病患者的BAL淋巴细胞中表达，而在10例正常肺实质患者中仅表达1例。 FasL protein was expressed in lymphocytes surrounding and within the granuloma. There was a significant correlation between the result of TUNEL and clinical course in patients with sarcoidosis. Apoptosis in epithelioid histiocytes and inflammatory cells seems to participate in the course of granulomatous inflammation. Further studies are needed to determine the role of Fas, FasL and other regulatory factors in apoptosis in the granulomatous inflammation in pulmonary sarcoidosis.