TY - JOUR T1 -健康人类志愿者暴露于臭氧后鼻灌洗液中抗氧化剂的消耗和补充动力学JF -欧洲呼吸杂志JO - Eur Respir J SP - 1429 LP - 1438 DO - 10.1183/09031936.99.13614399 VL - 13 IS - 6 AU - Mudway, IS AU - Blomberg, A AU - Frew, AJ AU - Holgate, ST AU - Sandstrom, T AU - Kelly，FJ Y1 - 1999/06/01 UR - //www.qdcxjkg.com/content/13/6/1429.abstract N2 -为了获得臭氧暴露期间人体呼吸道黏膜液(RTLFs)中发生的实时事件的信息，对13名在不同场合暴露于百万分之0.2臭氧和过滤空气(暴露2小时，间歇运动)的人类志愿者进行了连续鼻灌洗。在每次暴露的四个时间点进行鼻腔灌洗并采集血液样本:暴露前(Pre-E)、暴露后1小时(1h-E)、暴露后立即(0h-PE)和暴露后1小时(1h-PE)。暴露后1.5h (1.5h- pe)进行支气管黏膜活检。鼻RTLF中性粒细胞在03暴露期间或暴露后1.5小时不明显。此外，既存的中性粒细胞种群没有被激活。03暴露方案未改变气道通透性。连续灌洗导致RTLF抗坏血酸，还原性谷胱甘肽，细胞外超氧化物歧化酶和髓过氧化物酶在1h-E, 0h-PE和1.5h-PE相对于基线Pre-E值的显著冲洗。相反，RTLF尿酸(UA)、总蛋白和白蛋白浓度不显示冲洗动力学。在测试的抗氧化剂中，只有UA在03，浓度下明显减少，在1h-E时下降了6.22微mol x L(-1)，而在对照空气暴露期间下降了1.61微mol x L(-1) (p<0.01)。 The establishment of a new pseudo-steady-state concentration of RTLF UA (70% of Pre-E values) during the second hour of O3 exposure was coincident with a small but significant increase in plasma UA concentration (19.27 (O3) versus 1.95 micromol x L(-1) (air), p<0.05). These data demonstrate that inhalation of 0.2 parts per million 03 results in the depletion of nasal respiratory tract lining fluid uric acid and that this regional loss of uric acid leads to a small increase in plasma uric acid concentration. Whilst the reaction of uric acid with inspired 03 may confer protection locally, the role of upper airway uric acid as a sink for inhaled O3 is not supported by these findings. ER -