PT - 日刊文AU - Pokorski，男AU - 坂上，H AU - 近藤，H TI - 经典蛋白激酶C和在猫和大鼠颈动脉体DP的缺氧刺激诱导易位 - 2000年9月1 TA - 欧洲呼吸杂志PG- 459--463 VI - 16 IP - 3 4099 - 4100 //www.qdcxjkg.com/content/16/3/459.short - //www.qdcxjkg.com/content/16/3/459.full SO - 欧洲呼吸J2000 01九月;16 AB - 的存在，亚细胞分布，物种特异性和经典的蛋白的可能缺氧刺激诱导易位激酶C在颈动脉体进行了检查（cPKC）同工酶。颈动脉体从体内暴露于常氧或急性缺氧条件下的猫和鼠常氧解剖。对于同种型特异性的免疫组化单克隆抗血清PKCα的，PKCbetaI，PKCbetaII和PKCgamma使用。所述免疫反应由异硫氰酸荧光素（FITC）标记可视化。FITC /德克萨斯红双标记标本为cPKC同工酶使用/酪氨酸羟化酶证明化学感受器细胞定位的cPKC同工酶。使用激光扫描共聚焦图像技术检测免疫荧光。结果表明，PKCα的和PKCgamma的表达，但不PKCbeta在颈动脉体化学感受器细胞的细胞质同种型。为cPKC的化学感受器细胞定位提供了证据，双标记同种型检测。 The immunostaining was most intense in the periphery of the perikarya, the nuclear envelope and, occasionally, the nucleoplasm. No major differences were found in the immunolocalization of PKCalpha and PKCgamma under normoxic and hypoxic conditions or between species. However, the immunoreactivity tended to accumulate more in the peripheral cytoplasm and away from the nucleus in the hypoxic chemoreceptor cell. This study demonstrates the presence of classical protein kinase C enzymes in chemoreceptor cells. The intensity of the immunoreactivity may suggest a role for the classical protein kinase C signalling pathway in shaping the hypoxic response at the carotid body. However, this study failed to provide firm evidence of this.