%A Roca-Ferrer, J %A Mullol, J %A Lopez, E %A Xaubet, A %A Pujols, L %A Fernandez, JC %A Picado，局部抗炎药物对上皮细胞诱导的嗜酸性粒细胞存活和GM-CSF分泌的影响这种作用可能是由于促进嗜酸性粒细胞存活的上皮细胞产物的释放。我们研究了丙酸氟替卡松、布地奈德、二丙酸倍氯米松和nedocromil钠对粒细胞/巨噬细胞集落刺激因子(GM-CSF)释放的影响，以及对培养的鼻上皮细胞分泌物诱导的嗜酸性粒细胞存活的影响。人上皮细胞条件培养基(HECM)是由接受鼻矫正手术的健康受试者培养的上皮细胞产生的。从外周血中分离出密度均匀的嗜酸性粒细胞，分别与含或不含药物产生的HECM孵育。所有试验药物均抑制嗜酸性粒细胞存活，且反应呈剂量依赖性。丙酸氟替卡松的抑制效果最高(25%抑制浓度(IC25) 1x10(-9) M)，其次是布地奈德(IC25 3.3x10(-8) M)、二丙酸倍氯米松(IC25 1.5x10(-6) M)和奈多克龙奈钠(IC25 5x10(-6) M)。其次是布地奈德(IC25 2x10(-9) M)、二丙酸倍氯米松(IC25 13x10(-8) M)和奈多克龙钠(IC25 >10(-5) M)。p < 0.05)。 Topical anti-inflammatory drugs may decrease eosinophil survival by abrogating the promoting effect of epithelial cells. These drugs may exert part of their therapeutic effect by modulating GM-CSF release. The following rank of potency was observed: fluticasone propionate > budesonide > beclomethasone dipropionate > nedocromil sodium. The study of the interaction between epithelial cells and eosinophils may be a useful method for investigating and comparing the potency of topical drugs. %U //www.qdcxjkg.com/content/erj/10/7/1489.full.pdf