TY -的T1 nedocromil钠对人类的影响气道上皮cell-induced嗜酸性粒细胞趋化性和坚持人类内皮细胞体外JF -欧洲呼吸杂志》乔和J SP - 851 LP - 857欧元六世- 10 - 4盟作为礼尚往来,MM盟——Devalia杰盟-凯尔,OA盟——Rusznak C AU -卡尔德隆,米非盟- Sapsford RJ AU - Bayram, H AU -戴维斯,RJ Y1 - 1997/04/01 UR - //www.qdcxjkg.com/content/10/4/851.abstract N2 -尽管一些研究已经表明,长期治疗的哮喘患者nedocromil钠可降低气道高反应性和改善症状和肺功能,其影响的机制并不清楚。我们已经调查了nedocromil钠对嗜酸性粒细胞趋化作用的影响,嗜酸性粒细胞坚持人类内皮细胞和释放可溶性细胞间粘附molecule-1 (sICAM-1)内皮细胞,引起人类支气管上皮细胞收集的条件培养基培养。Conditioned medium significantly increased eosinophil chemotaxis from a baseline median value of 2.1 (range 1.9-4.5) cells-high power field(-1) (HPF) to 10.5 (range 7.8-12.3) cells-HPF(-1) (p<0.05). Similarly, conditioned medium significantly increased eosinophil adherence to endothelial cells from a baseline value of 9 (range 8-12)% to 23 (range 21-30)% (p<0.05). Nedocromil sodium, at 10(-5) M concentration, significantly attenuated the eosinophil chemotaxis and adherence induced by conditioned medium. Conditioned medium also significantly increased the release of sICAM-1 from endothelial cells, from a baseline value of 11.5 (range 8.1-15.4) pg x microg(-1) protein to 67.6 (range 55.6-73.5) pg x microg(-1) protein (p<0.05). This was significantly attenuated by anti-tumour necrosis factor-alpha (TNF-alpha), anti-interleukin-1beta (IL-1beta) and 10(-5) M nedocromil sodium. These findings suggest that human bronchial epithelial cell-derived mediators may potentiate eosinophil activity, and that this can be modulated by nedocromil sodium, suggesting a possible mechanism underlying its anti-inflammatory effect. ER -