TY - JOUR T1 -碳酸酸酶IX抗原在非小细胞肺癌肿瘤前恶性病变中的鉴别JF -欧洲呼吸杂志JO - Eur Respir J SP - 806 LP - 811 VL - 14 IS - 4 AU - Vermylen, P AU - Roufosse, C AU - Burny, A AU - Verhest, A AU - Bosschaerts, T AU - Pastorekova, S AU - Ninane, V AU - Sculier，MaTu间隔(MN)/碳酸酐酶(CA) IX肿瘤相关抗原是一种在肠道中正常表达的蛋白质，属于碳酸酐酶家族(CA IX)。它已在肿瘤细胞系和一些实体肿瘤中检测到，包括宫颈癌、食管癌和透明细胞肾癌。本研究测定了65例治疗目的切除的原发性非小细胞肺癌和38例支气管癌前病变、原位癌或微浸润性癌以及正常支气管组织中MN/CA IX的表达。当冷冻材料可用时，用免疫组化和Western blot分析检测MN/CA IX的存在。52/65(80%)的肿瘤样本免疫染色呈阳性。染色多为局部性而非弥漫性。阳性肿瘤中染色细胞的比例变化很大，在1-85%之间。免疫染色模式主要是细胞质和膜强化(87%)。强度以强为主(69%)。对8个样本的Western blot分析证实了肿瘤中这种蛋白质的存在。 All the morphologically normal epithelia, except in close vicinity of tumours in some cases, as well as the preneoplastic bronchial lesions (basal cell hyperplasia, metaplasia and dysplasia) were immunonegative for MN/CA IX expression. In contrast, carcinoma in situ and microinvasive epithelioma showed the presence of MN-immunopositive tumoural cells in 5/7 and 4/5 of the samples, respectively. These data suggest that MN/CA IX is a useful marker for the differentiation between preneoplastic lesions and bronchial non-small cell lung cancer in the lung. ER -