Ty -jour t1-使用光学亮点JF的肺曲霉病的诊断 - 欧洲呼吸杂志-Moller,K au -Ruchel,R Y1-2000/02/01 Ur -http://www.qdcxjkg.com/content/15/2/407.abstract N2-侵入性肺曲霉病(IPA)越来越多地识别免疫功能低下的患者,但早期诊断仍然是一个问题。临床标本中的真菌元素可以用光学亮丽器直接染色。引起的荧光的高强度可以快速可靠的显微镜筛选。在本研究中,作者旨在验证这种方法。前瞻性地评估了培养物并用光学亮点Blackophor-p-flussig染色的所有试物(n = 94)的支气管分泌物或胸膜液(n = 94)的所有标本。使用临床,培养和放射学数据的组合以及活检(n = 3)或尸检(n = 3),使用了13名患者的17例标本诊断IPA。IPA的总体发生率为3.3%。IPA患者中有9名死亡(死亡率= 69%)。 Staining with the optical brightener and consecutive microscopic screening took 9+/-3 min. For the diagnosis of invasive aspergillosis, sensitivity was 0.88 and specificity was 0.99. Using culture, sensitivity was 0.76 and specificity was 0.99. In conclusion, direct examination of clinical specimens using the optical brightener has a high diagnostic potential for the diagnosis of invasive pulmonary aspergillosis. The reliability, simplicity and speed of the method render it suitable for routine diagnostic work. ER -