ty -jour t1-从人支气管型和功能上独特的巨噬细胞亚群中的分离与人支气管肺泡灌洗jf -usure呼吸杂志 - 欧洲呼吸杂志-Poulter，LW Y1-1992/06/01 UR -http：//www.qdcxjkg.com/content/5/6/717.abstract N2-使用下呼吸道肺泡（AM）从下呼吸道获取肺泡巨噬细胞（AM）健康的正常志愿者。然后将针对巨噬细胞决定因素的单克隆抗体（MOAB）探针与密度分离技术结合使用，以识别和分离出AM池的三个相对均匀的亚群。先前已显示使用的MoABS RFD1和RFD7分别在正常组织中分别区分“树突状”细胞和成熟的巨噬细胞。除了这两个表型不同的AM子集（RFD1+D7-和RFD1-D7+AM）外，分离了第三个AM亚群，似乎表达了这两个标记（RFD1+D7+）。这三个分离的巨噬细胞亚群在形态上都是相似的，但在表面受体表达，酶含量和生理学方面都显示出明显的差异。分离的RFD1+D7- AM（“树突状”细胞的表型）不粘附在玻璃上，C3B和FCR1受体的表达弱，纤连蛋白纤维蛋白含量低和溶酶体活性低；这些细胞中只有一小部分表现出吞噬作用。其他两个隔离的AM子集粘附在玻璃，表达的C3B和FCR1受体上，具有高纤连蛋白和酸性磷酸酶含量，并且绝大多数表现出吞噬能力。 qualitative and quantitative differences in these features existed between the two AM subtypes. Furthermore, a diverse spectrum of hexose monophosphate shunt activity was observed throughout all three AM subpopulations, with the highest activity being recorded in the non-adherent AM. These data support the concept of a dynamic heterogeneity within the AM population. The variation in surface antigen expression and physiological capabilities observed amongst the three isolated AM subsets implies the presence of functionally distinct AM within the human lung, which, during steady-state conditions, may be critically balanced under the influence of stimuli in their local microenvironment. In support, proportional and functional shifts have been witnessed amongst these three AM subpopulations with the advent of disease. ER -